Overview

  • Product name

  • Description

    Rabbit polyclonal to ARPC1B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ARPC1B aa 272-332.
    Database link: NP_005711.1

  • Positive control

    • HeLa, 293T and NIH3T3 cell lysates
  • General notes

     This product was previously labelled as ARP2/3 subunit 1B

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab99314 was affinity purified using an epitope specific to ARPC1B immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

Images

  • ab99314 at 1 µg/ml detecting ARPC1B in HeLa whole cell lysate by WB following IP.
    Lane 1: ab99314 at 3µg/mg of lysate.
    Lane 2: IP with an antibody which recognizes an downstream epitope of ARPC1B.
    Lane 3: control IgG.
    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
    Detection: Chemiluminescence with an exposure time of 3 seconds.

  • All lanes : Anti-ARPC1B antibody (ab99314) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : NIH3T3 cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 41 kDa


    Exposure time: 3 minutes

References

This product has been referenced in:

  • Bertier L  et al. Nanobodies targeting cortactin proline rich, helical and actin binding regions downregulate invadopodium formation and matrix degradation in SCC-61 cancer cells. Biomed Pharmacother 102:230-241 (2018). Read more (PubMed: 29567535) »
  • Bertier L  et al. Inhibitory cortactin nanobodies delineate the role of NTA- and SH3-domain-specific functions during invadopodium formation and cancer cell invasion. FASEB J 31:2460-2476 (2017). Read more (PubMed: 28235780) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Testis)
Permeabilization
Yes - 2% Triton X 100
Specification
Testis
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 06 2016

Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Testis)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Specification
Testis
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 05 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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