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    arpc2-antibody-ab11798.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly
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Anti-ARPC2 antibody (ab11798)

  • Datasheet
  • SDS
Submit a review Submit a question References (4)

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Western blot - Anti-ARPC2 antibody (ab11798)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody (ab11798)
  • Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)
  • Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)

Key features and details

  • Goat polyclonal to ARPC2
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-ARPC2 antibody [EPR8533] (ab133315)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-ARPC2 antibody
    See all ARPC2 primary antibodies
  • Description

    Goat polyclonal to ARPC2
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognise both reported isoforms as represented by NP_005722 and NP_690601.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide: EMKTITGKTFSSR, corresponding to amino acids 288-300 of Human ARPC2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Caco-2, HeLa and Jurkat cell lysates. IHC-P: Human Muscle lysates. ICC/IF: HeLa and A431 cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human ARPC2 protein (ab140555)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Rat
Zebrafish
Application Abreviews Notes
IHC-P
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 34 kDa.

A 1 hour primary incubation is recommended for this product.

ICC/IF
Use a concentration of 10 µg/ml.

PubMed ID: 22021442

Notes
IHC-P
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 34 kDa.

A 1 hour primary incubation is recommended for this product.

ICC/IF
Use a concentration of 10 µg/ml.

PubMed ID: 22021442

Target

  • Function

    Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
  • Sequence similarities

    Belongs to the ARPC2 family.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection.
  • Target information above from: UniProt accession O15144 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10109 Human
    • Entrez Gene: 301511 Rat
    • Entrez Gene: 327068 Zebrafish
    • Omim: 604224 Human
    • SwissProt: O15144 Human
    • SwissProt: P85970 Rat
    • Unigene: 529303 Human
    • Unigene: 93317 Rat
    • Alternative names

      • Actin related protein 2/3 complex subunit 2 34kDa antibody
      • Actin related protein 2/3 complex subunit 2 antibody
      • Actin-related protein 2/3 complex subunit 2 antibody
      • ARC 34 antibody
      • ARC34 antibody
      • Arp2/3 complex 34 kDa subunit antibody
      • ARP2/3 protein compex subunit 34 antibody
      • ARPC 2 antibody
      • Arpc2 antibody
      • ARPC2_HUMAN antibody
      • p34 Arc antibody
      • p34-ARC antibody
      • p34Arc antibody
      • PNAS 139 antibody
      • PNAS139 antibody
      • PRO 2446 antibody
      • PRO2446 antibody
      see all

    Images

    • Western blot - Anti-ARPC2 antibody (ab11798)
      Western blot - Anti-ARPC2 antibody (ab11798)
      Lane 1 : Anti-ARPC2 antibody (ab11798) at 0.5 µg/ml
      Lanes 2-3 : Anti-ARPC2 antibody (ab11798) at 0.1 µg/ml

      Lane 1 : CACO-2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 35 µg per lane.

      Observed band size: 34-35 kDa why is the actual band size different from the predicted?



      Detected by chemiluminescence.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody (ab11798)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody (ab11798)
      ab11798 (3.8 µg/ml) staining ARPC2 in paraffin-embedded Human spleen tissue following steamed antigen retrieval with citrate buffer (pH6).
    • Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)
      Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)

      Immunocytochemistry/ Immunofluorescence analysis of A431 cells labeling ARPC2 with ab11798 at 10 µg/mL (1 hour) showing cytoplasmic statining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

    • Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)
      Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)

      Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling ARPC2 with ab11798 at 10 µg/mL (1 hour) showing cytoplasmic statining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab11798? Please let us know so that we can cite the reference in this datasheet.

    ab11798 has been referenced in 4 publications.

    • Cheng Z  et al. ARPC2 promotes breast cancer proliferation and metastasis. Oncol Rep 41:3189-3200 (2019). PubMed: 31002363
    • Xia P  et al. WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner. J Exp Med 211:2119-34 (2014). PubMed: 25225459
    • Chauhan BK  et al. Balanced Rac1 and RhoA activities regulate cell shape and drive invagination morphogenesis in epithelia. Proc Natl Acad Sci U S A 108:18289-94 (2011). ICC/IF ; Mouse . PubMed: 22021442
    • Li Y  et al. The profilin:actin complex localizes to sites of dynamic actin polymerization at the leading edge of migrating cells and pathogen-induced actin tails. Eur J Cell Biol 87:893-904 (2008). PubMed: 18707793

    Customer reviews and Q&As

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