Anti-ARPC2 antibody (ab11798)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-ARPC2 antibody
See all ARPC2 primary antibodies -
Description
Goat polyclonal to ARPC2 -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms as represented by NP_005722 and NP_690601. -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Zebrafish
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Immunogen
Synthetic peptide: EMKTITGKTFSSR, corresponding to amino acids 288-300 of Human ARPC2.
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Positive control
- WB: Caco-2, HeLa and Jurkat cell lysates. IHC-P: Human Muscle lysates. ICC/IF: HeLa and A431 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab11798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 34 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. PubMed ID: 22021442 |
Target
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Function
Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. -
Sequence similarities
Belongs to the ARPC2 family. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection. - Information by UniProt
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Database links
- Entrez Gene: 10109 Human
- Entrez Gene: 76709 Mouse
- Entrez Gene: 301511 Rat
- Entrez Gene: 327068 Zebrafish
- Omim: 604224 Human
- SwissProt: O15144 Human
- SwissProt: Q9CVB6 Mouse
- SwissProt: P85970 Rat
see all -
Alternative names
- Actin related protein 2/3 complex subunit 2 34kDa antibody
- Actin related protein 2/3 complex subunit 2 antibody
- Actin-related protein 2/3 complex subunit 2 antibody
see all
Images
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Western blot - Anti-ARPC2 antibody (ab11798)Lane 1 : Anti-ARPC2 antibody (ab11798) at 0.5 µg/ml
Lanes 2-3 : Anti-ARPC2 antibody (ab11798) at 0.1 µg/ml
Lane 1 : CACO-2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 35 µg per lane.
Observed band size: 34-35 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody (ab11798)ab11798 (3.8 µg/ml) staining ARPC2 in paraffin-embedded Human spleen tissue following steamed antigen retrieval with citrate buffer (pH6). -
Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)Immunocytochemistry/ Immunofluorescence analysis of A431 cells labeling ARPC2 with ab11798 at 10 µg/mL (1 hour) showing cytoplasmic statining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
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Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling ARPC2 with ab11798 at 10 µg/mL (1 hour) showing cytoplasmic statining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Protocols
Datasheets and documents
References
This product has been referenced in:
- Cheng Z et al. ARPC2 promotes breast cancer proliferation and metastasis. Oncol Rep 41:3189-3200 (2019). Read more (PubMed: 31002363) »
- Xia P et al. WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner. J Exp Med 211:2119-34 (2014). Read more (PubMed: 25225459) »
