Anti-ARPC2 antibody (ab11798)
- Datasheet
- References (3)
- Protocols
Overview
-
Product nameAnti-ARPC2 antibody
See all ARPC2 primary antibodies -
DescriptionGoat polyclonal to ARPC2
-
Host speciesGoat
-
SpecificityThis antibody is expected to recognise both reported isoforms as represented by NP_005722 and NP_690601.
-
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
-
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Zebrafish
-
Immunogen
Synthetic peptide: EMKTITGKTFSSR, corresponding to amino acids 288-300 of Human ARPC2.
-
Positive control
- Hela and Human Muscle lysates.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab11798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 34 kDa.Can be blocked with Human ARPC2 peptide (ab23207). | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 22021442 |
Target
-
FunctionFunctions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
-
Sequence similaritiesBelongs to the ARPC2 family.
-
Cellular localizationCytoplasm > cytoskeleton. Cell projection.
- Information by UniProt
-
Database links
- Entrez Gene: 10109 Human
- Entrez Gene: 76709 Mouse
- Entrez Gene: 301511 Rat
- Entrez Gene: 327068 Zebrafish
- Omim: 604224 Human
- SwissProt: O15144 Human
- SwissProt: Q9CVB6 Mouse
- SwissProt: P85970 Rat
see all -
Alternative names
- Actin related protein 2/3 complex subunit 2 34kDa antibody
- Actin related protein 2/3 complex subunit 2 antibody
- Actin-related protein 2/3 complex subunit 2 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody (ab11798)ab11798 (3.8 µg/ml) staining ARPC2 in paraffin-embedded Human spleen tissue following steamed antigen retrieval with citrate buffer (pH6).
Protocols
Datasheets and documents
References
This product has been referenced in:
- Xia P et al. WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner. J Exp Med 211:2119-34 (2014). Read more (PubMed: 25225459) »
- Chauhan BK et al. Balanced Rac1 and RhoA activities regulate cell shape and drive invagination morphogenesis in epithelia. Proc Natl Acad Sci U S A 108:18289-94 (2011). ICC/IF ; Mouse . Read more (PubMed: 22021442) »
