Anti-ARPC2 antibody (ab11798)
Key features and details
- Goat polyclonal to ARPC2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ARPC2 antibody
See all ARPC2 primary antibodies -
Description
Goat polyclonal to ARPC2 -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms as represented by NP_005722 and NP_690601. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide: EMKTITGKTFSSR, corresponding to amino acids 288-300 of Human ARPC2.
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Positive control
- WB: Caco-2, HeLa and Jurkat cell lysates. IHC-P: Human Muscle lysates. ICC/IF: HeLa and A431 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Rat
Zebrafish
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 34 kDa.
A 1 hour primary incubation is recommended for this product. |
|
| ICC/IF |
Use a concentration of 10 µg/ml.
PubMed ID: 22021442 |
| Notes |
|---|
|
IHC-P
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 34 kDa. A 1 hour primary incubation is recommended for this product. |
|
ICC/IF
Use a concentration of 10 µg/ml. PubMed ID: 22021442 |
Target
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Function
Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. -
Sequence similarities
Belongs to the ARPC2 family. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection. - Information by UniProt
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Database links
- Entrez Gene: 10109 Human
- Entrez Gene: 301511 Rat
- Entrez Gene: 327068 Zebrafish
- Omim: 604224 Human
- SwissProt: O15144 Human
- SwissProt: P85970 Rat
- Unigene: 529303 Human
- Unigene: 93317 Rat
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Alternative names
- Actin related protein 2/3 complex subunit 2 34kDa antibody
- Actin related protein 2/3 complex subunit 2 antibody
- Actin-related protein 2/3 complex subunit 2 antibody
see all
Images
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Western blot - Anti-ARPC2 antibody (ab11798)Lane 1 : Anti-ARPC2 antibody (ab11798) at 0.5 µg/ml
Lanes 2-3 : Anti-ARPC2 antibody (ab11798) at 0.1 µg/ml
Lane 1 : CACO-2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 35 µg per lane.
Observed band size: 34-35 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody (ab11798)ab11798 (3.8 µg/ml) staining ARPC2 in paraffin-embedded Human spleen tissue following steamed antigen retrieval with citrate buffer (pH6). -
Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)Immunocytochemistry/ Immunofluorescence analysis of A431 cells labeling ARPC2 with ab11798 at 10 µg/mL (1 hour) showing cytoplasmic statining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
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Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody (ab11798)Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling ARPC2 with ab11798 at 10 µg/mL (1 hour) showing cytoplasmic statining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 at 2 µg/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab11798 has been referenced in 4 publications.
- Cheng Z et al. ARPC2 promotes breast cancer proliferation and metastasis. Oncol Rep 41:3189-3200 (2019). PubMed: 31002363
- Xia P et al. WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner. J Exp Med 211:2119-34 (2014). PubMed: 25225459
- Chauhan BK et al. Balanced Rac1 and RhoA activities regulate cell shape and drive invagination morphogenesis in epithelia. Proc Natl Acad Sci U S A 108:18289-94 (2011). ICC/IF ; Mouse . PubMed: 22021442
- Li Y et al. The profilin:actin complex localizes to sites of dynamic actin polymerization at the leading edge of migrating cells and pathogen-induced actin tails. Eur J Cell Biol 87:893-904 (2008). PubMed: 18707793
Customer reviews and Q&As
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