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    arpc2-antibody-ab14378.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly
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Anti-ARPC2 antibody (ab14378)

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Western blot - Anti-ARPC2 antibody (ab14378)

    Key features and details

    • Rabbit polyclonal to ARPC2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-ARPC2 antibody
      See all ARPC2 primary antibodies
    • Description

      Rabbit polyclonal to ARPC2
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide within Human ARPC2 aa 285-298 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
      Sequence:

      EKKEMKTITGKTFS


      Database link: O15144
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • WB: A431 cell lysate.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.05% Sodium azide
      Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin Assembly

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • A-431 whole cell lysate (ab7909)
    • Recombinant Protein

      • Recombinant Human ARPC2 protein (ab140555)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab14378 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 34 kDa.
    Notes
    WB
    Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 34 kDa.

    Target

    • Function

      Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
    • Sequence similarities

      Belongs to the ARPC2 family.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cell projection.
    • Target information above from: UniProt accession O15144 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10109 Human
      • Omim: 604224 Human
      • SwissProt: O15144 Human
      • Unigene: 529303 Human
      • Alternative names

        • Actin related protein 2/3 complex subunit 2 34kDa antibody
        • Actin related protein 2/3 complex subunit 2 antibody
        • Actin-related protein 2/3 complex subunit 2 antibody
        • ARC 34 antibody
        • ARC34 antibody
        • Arp2/3 complex 34 kDa subunit antibody
        • ARP2/3 protein compex subunit 34 antibody
        • ARPC 2 antibody
        • Arpc2 antibody
        • ARPC2_HUMAN antibody
        • p34 Arc antibody
        • p34-ARC antibody
        • p34Arc antibody
        • PNAS 139 antibody
        • PNAS139 antibody
        • PRO 2446 antibody
        • PRO2446 antibody
        see all

      Images

      • Western blot - Anti-ARPC2 antibody (ab14378)
        Western blot - Anti-ARPC2 antibody (ab14378)
        Anti-ARPC2 antibody (ab14378) at 1 µg/ml + A431 (Human epidermoid carcinoma cell line) cell lysate in RIPA buffer

        Secondary
        Goat anti-rabbit IgG (HRP)

        Predicted band size: 34 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab14378? Please let us know so that we can cite the reference in this datasheet.

      ab14378 has not yet been referenced specifically in any publications.

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