Key features and details
- Rabbit polyclonal to ARPC2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human, Zebrafish
- Isotype: IgG
Product nameAnti-ARPC2 antibody
See all ARPC2 primary antibodies
DescriptionRabbit polyclonal to ARPC2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human, Zebrafish
Predicted to work with: Cow
Recombinant fragment, corresponding to a region within amino acids 14-277 of Human ARPC2 (NP_005722).
- 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji and NIH 3T3 whole cell lysates. HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 34 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionFunctions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
Sequence similaritiesBelongs to the ARPC2 family.
Cellular localizationCytoplasm > cytoskeleton. Cell projection.
- Information by UniProt
- Actin related protein 2/3 complex subunit 2 34kDa antibody
- Actin related protein 2/3 complex subunit 2 antibody
- Actin-related protein 2/3 complex subunit 2 antibody
ab96779 at 1/200 dilution staining ARPC2 in paraformaldehyde-fixed HeLa cells by immunofluorescence. The lower image shows ab96779 merged with a DNA probe.
Anti-ARPC2 antibody (ab96779) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 34 kDa
10% SDS PAGE
All lanes : Anti-ARPC2 antibody (ab96779) at 1/500 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa S3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
12% SDS PAGE
ab96779 has been referenced in 3 publications.
- Zhang Y et al. Arp2/3 complex controls T cell homeostasis by maintaining surface TCR levels via regulating TCR+endosome trafficking. Sci Rep 7:8952 (2017). PubMed: 28827576
- Qian X et al. Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes. Endocrinology 154:1907-20 (2013). Rat . PubMed: 23546604
- Qian X et al. Breast cancer resistance protein regulates apical ectoplasmic specialization dynamics stage specifically in the rat testis. Am J Physiol Endocrinol Metab 304:E757-69 (2013). WB, IF . PubMed: 23403943