RecombinantRabMAb

Recombinant Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)
  • Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)
  • Immunoprecipitation - Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)
  • Flow Cytometry - Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)
  • Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8533] to ARPC2 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ARPC2 antibody [EPR8533] - BSA and Azide free
    See all ARPC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8533] to ARPC2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: ICC/IF, IHC-P, IP, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human ARPC2 aa 250-350. The exact sequence is proprietary.
    Database link: O15144

  • Positive control

    • IHC-P: Human kidney, tonsil and gastric carcinoma tissue; ICC/IF: HeLa cells; WB: HEK-293, Mouse heart, HT-1080, Jurkat, HeLa, Neuro-2a, and Rat heart lysates IP: HEK-293 lysate

  • General notes

    Ab248468 is the carrier-free version of ab133315. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248468 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab248468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.

Target

  • Function

    Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.

  • Sequence similarities

    Belongs to the ARPC2 family.

  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Actin related protein 2/3 complex subunit 2 34kDa antibody
    • Actin related protein 2/3 complex subunit 2 antibody
    • Actin-related protein 2/3 complex subunit 2 antibody
    • ARC 34 antibody
    • ARC34 antibody
    • Arp2/3 complex 34 kDa subunit antibody
    • ARP2/3 protein compex subunit 34 antibody
    • ARPC 2 antibody
    • Arpc2 antibody
    • ARPC2_HUMAN antibody
    • p34 Arc antibody
    • p34-ARC antibody
    • p34Arc antibody
    • PNAS 139 antibody
    • PNAS139 antibody
    • PRO 2446 antibody
    • PRO2446 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric carcinoma tissue sections labeling ARPC2 with purified ab133315 at 1/2000 dilution (0.25 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133315)
  • Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)
    Immunocytochemistry/ Immunofluorescence - Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ARPC2 with purified ab133315 at 1:100 dilution (5.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunoprecipitation - Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)
    Immunoprecipitation - Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)
    ab133315 (purified ) at 1/30 dilution (2 µg) immunoprecipitating ARPC2 in HEK-293 whole cell lysate.
    Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab133315 & HEK-293 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133315 in HEK-293 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133315)
  • Flow Cytometry - Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)
    Flow Cytometry - Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ARPC2 with purified ab133315 at 1/50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133315)
  • Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)
    Anti-ARPC2 antibody [EPR8533] - BSA and Azide free (ab248468)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab248468? Please let us know so that we can cite the reference in this datasheet.

ab248468 has not yet been referenced specifically in any publications.

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