Key features and details
- Goat polyclonal to ARPC4
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARPC4 antibody
See all ARPC4 primary antibodies
DescriptionGoat polyclonal to ARPC4
Specificityab80502 is expected to recognise isoform a (NP_005709.1).
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Zebrafish
- Human lymph node lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab80502 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab80502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
Peptide ELISA: antibody detection limit dilution 1/64000.
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 20 kDa.
1 hour primary incubation is recommended for this product.
FunctionFunctions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
Sequence similaritiesBelongs to the ARPC4 family.
Cellular localizationCytoplasm > cytoskeleton. Cell projection.
- Information by UniProt
- Actin related protein 2/3 complex subunit 4 antibody
- Actin related protein 2/3 complex subunit 4 20 kD antibody
- Actin related protein 2/3 complex subunit 4 20kDa antibody
Anti-ARPC4 antibody (ab80502) at 0.03 µg/ml + human lymph node lysate (in RIPA buffer) at 35 µg
Predicted band size: 20 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab80502 has been referenced in 1 publication.
- Fu X et al. Doublecortin (Dcx) family proteins regulate filamentous actin structure in developing neurons. J Neurosci 33:709-21 (2013). PubMed: 23303949