Recombinant
RabMAb

Anti-ARPC5L antibody [EPR10274] (ab169763)

Overview

  • Product name
    Anti-ARPC5L antibody [EPR10274]
    See all ARPC5L primary antibodies
  • Description
    Rabbit monoclonal [EPR10274] to ARPC5L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ARPC5L.
    Database link: Q9BPX5

  • Positive control
    • U87-MG, MCF7, MOLT4 and fetal brain lysates, Human heart tissue, MCF7 cells, MOLT4 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10274
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P 1/250 - 1/500.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Flow Cytometrical analysis of permeabilized MOLT4 cells with ab169763 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

  • Immunofluorescent analysis of MCF7 cells labeling ARPC5 with ab169763 at 1/250 (red) with DAPI staining (blue).

  • Immunohistochemical analysis of paraffin embedded Human heart tissue labeling ARPC5L with ab169763 at 1/250.

  • All lanes : Anti-ARPC5L antibody [EPR10274] (ab169763) at 1/1000 dilution

    Lane 1 : U87-MG lysate
    Lane 2 : MCF7 lysate
    Lane 3 : MOLT4 lysate
    Lane 4 : Fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 17 kDa

References

ab169763 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab169763.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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