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  1. Link

    arpc5p16-arc-antibody-ep1551y-ab51243.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243)

  • Datasheet
  • SDS
Reviews (5)Q&A (2)References (8)

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Western blot - Anti-p16 ARC antibody [EP1551Y] (ab51243)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p16 ARC antibody [EP1551Y] (ab51243)
  • Immunocytochemistry/ Immunofluorescence - Anti-p16 ARC antibody [EP1551Y] (ab51243)
  • Immunoprecipitation - Anti-p16 ARC antibody [EP1551Y] (ab51243)
  • Western blot - Anti-p16 ARC antibody [EP1551Y] (ab51243)
  • Western blot - Anti-p16 ARC antibody [EP1551Y] (ab51243)
  • Western blot - Anti-p16 ARC antibody [EP1551Y] (ab51243)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p16 ARC antibody [EP1551Y] (ab51243)
  • Anti-p16 ARC antibody [EP1551Y] (ab51243)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1551Y] to ARPC5/p16 ARC
  • Suitable for: ICC/IF, IP, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-ARPC5/p16 ARC antibody [EP1551Y]
    See all ARPC5/p16 ARC primary antibodies
  • Description

    Rabbit monoclonal [EP1551Y] to ARPC5/p16 ARC
  • Host species

    Rabbit
  • Specificity

    This antibody is specific to the p16 ARC protein, database link: O15511. There is no observed cross reactivity to the CDKN2A/p16INK4a protein.

  • Tested applications

    Suitable for: ICC/IF, IP, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal, mouse and rat brain tissue lysates and HeLa, HepG2, SKBR-3 and MCF-7 cell lysates. IHC-P: Human uterus adenocarcinoma and spleen tissues. ICC/IF: Neuro-2a cells. IP: Human fetal brain tissue lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1551Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly

Associated products

  • Alternative Versions

    • Anti-ARPC5/p16 ARC antibody [EP1551Y] - BSA and Azide free (ab220800)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
    • Mouse brain tissue lysate - total protein (ab30151)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50 - 1/100.
IP
1/20 - 1/70.
WB (5)
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

We have obtained results that indicate IHC-P is unsuitable for mouse and rat species.  

Notes
ICC/IF
1/50 - 1/100.
IP
1/20 - 1/70.
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

We have obtained results that indicate IHC-P is unsuitable for mouse and rat species.  

Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
  • Sequence similarities

    Belongs to the ARPC5 family.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection.
  • Target information above from: UniProt accession O15511 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10092 Human
    • Entrez Gene: 67771 Mouse
    • Entrez Gene: 360854 Rat
    • Omim: 604227 Human
    • SwissProt: O15511 Human
    • SwissProt: Q9CPW4 Mouse
    • SwissProt: Q4KLF8 Rat
    • Unigene: 518609 Human
    • Unigene: 288974 Mouse
    • Unigene: 1639 Rat
    see all
  • Alternative names

    • Actin related protein 2/3 complex subunit 5 (16 kD) antibody
    • Actin related protein 2/3 complex subunit 5 antibody
    • Actin related protein 2/3 complex, subunit 5 16kDa antibody
    • Actin-related protein 2/3 complex subunit 5 antibody
    • ARC16 antibody
    • Arp2/3 complex 16 kDa subunit antibody
    • Arp2/3 protein complex subunit p16 antibody
    • ARPC 5 antibody
    • Arpc5 antibody
    • ARPC5_HUMAN antibody
    • dJ127C7.3 antibody
    • MGC88523 antibody
    • p16 Arc antibody
    • p16-ARC antibody
    • RP1 127C7.3 antibody
    see all

Images

  • Western blot - Anti-p16 ARC antibody [EP1551Y] (ab51243)
    Western blot - Anti-p16 ARC antibody [EP1551Y] (ab51243)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: p16 ARC knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)

     

    Lanes 1 - 4: Merged signal (red and green). Green - ab51243 observed at 16 kDa. Red - loading control, ab8245, observed at 37 kDa.

     

    ab51243 was shown to specifically react with p16 ARC when p16 ARC knockout samples were used. Wild-type and p16 ARC knockout samples were subjected to SDS-PAGE. Ab51243 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p16 ARC antibody [EP1551Y] (ab51243)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p16 ARC antibody [EP1551Y] (ab51243)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling p16 ARC with purified ab51243 at a dilution of 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-p16 ARC antibody [EP1551Y] (ab51243)
    Immunocytochemistry/ Immunofluorescence - Anti-p16 ARC antibody [EP1551Y] (ab51243)

    Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling p16 ARC with purified ab51243 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Immunoprecipitation - Anti-p16 ARC antibody [EP1551Y] (ab51243)
    Immunoprecipitation - Anti-p16 ARC antibody [EP1551Y] (ab51243)

    ab51243 (purified) at a dilution of 1/20 immunoprecipitating p16 ARC in human fetal brain tissue lysate.

    Lane 1 (input): Human fetal brain tissue lysate (10µg)

    Lane 2 (+): ab51243 + human fetal brain tissue lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51243 in human fetal brain tissue lysate.

    For western blotting, a HRP-conjugated anti-rabbit specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-p16 ARC antibody [EP1551Y] (ab51243)
    Western blot - Anti-p16 ARC antibody [EP1551Y] (ab51243)
    All lanes : Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/5000 dilution (purified)

    Lane 1 : HeLa whole cell lysate
    Lane 2 : SKBR-3 whole cell lysate
    Lane 3 : MCF-7 whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 16 kDa
    Observed band size: 16 kDa



    Blocking and dilution buffer: 5% NFDM/TBST
  • Western blot - Anti-p16 ARC antibody [EP1551Y] (ab51243)
    Western blot - Anti-p16 ARC antibody [EP1551Y] (ab51243)
    All lanes : Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/5000 dilution (purified)

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 16 kDa
    Observed band size: 16 kDa



    Blocking and dilution buffer: 5% NFDM/TBST
  • Western blot - Anti-p16 ARC antibody [EP1551Y] (ab51243)
    Western blot - Anti-p16 ARC antibody [EP1551Y] (ab51243)
    All lanes : Anti-ARPC5/p16 ARC antibody [EP1551Y] (ab51243) at 1/2000 dilution (unpurified)

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 16 kDa



    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p16 ARC antibody [EP1551Y] (ab51243)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p16 ARC antibody [EP1551Y] (ab51243)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus adenocarcinoma tissue labelling p16 ARC with unpurified ab51243 at a dilution of 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-p16 ARC antibody [EP1551Y] (ab51243)
    Anti-p16 ARC antibody [EP1551Y] (ab51243)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (8)

Publishing research using ab51243? Please let us know so that we can cite the reference in this datasheet.

ab51243 has been referenced in 8 publications.

  • Indra I  et al. Sensing Actin Dynamics through Adherens Junctions. Cell Rep 30:2820-2833.e3 (2020). PubMed: 32101754
  • Wang Y  et al. 27-Hydroxycholesterol Alters Synaptic Structural and Functional Plasticity in Hippocampal Neuronal Cultures. J Neuropathol Exp Neurol 78:238-247 (2019). PubMed: 30753597
  • Choi J  et al. Pimozide suppresses cancer cell migration and tumor metastasis through binding to ARPC2, a subunit of the Arp2/3 complex. Cancer Sci 110:3788-3801 (2019). PubMed: 31571309
  • Moriya Y  et al. Tumor suppressive microRNA-133a regulates novel molecular networks in lung squamous cell carcinoma. J Hum Genet 57:38-45 (2012). PubMed: 22089643
  • Kinoshita T  et al. Actin-related protein 2/3 complex subunit 5 (ARPC5) contributes to cell migration and invasion and is directly regulated by tumor-suppressive microRNA-133a in head and neck squamous cell carcinoma. Int J Oncol 40:1770-8 (2012). PubMed: 22378351
  • Chang YF  et al. Interaction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation. Proc Natl Acad Sci U S A 109:5750-5 (2012). ICC/IF ; Mouse . PubMed: 22447776
  • Hubert T  et al. Actin and Arp2/3 localize at the centrosome of interphase cells. Biochem Biophys Res Commun 404:153-8 (2011). ICC/IF ; Human . PubMed: 21108927
  • Schmidt S  et al. Kindlin-3-mediated signaling from multiple integrin classes is required for osteoclast-mediated bone resorption. J Cell Biol 192:883-97 (2011). ICC/IF ; Mouse . PubMed: 21357746

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-p16 ARC antibody [EP1551Y]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (RPE1 cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
RPE1 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 10 2021

Western blot abreview for Anti-p16 ARC antibody [EP1551Y]

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain and Spleen)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Brain and Spleen
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ester Canto Puig

Verified customer

Submitted Jul 04 2016

Western blot abreview for Anti-p16 ARC antibody [EP1551Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (mouse lung)
Specification
mouse lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 27 2015

Western blot abreview for Anti-p16 ARC antibody [EP1551Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7)
Loading amount
100000 cells
Specification
MCF7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 17 2012

Western blot abreview for Anti-p16 ARC antibody [EP1551Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
5 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 15 2010

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