Product nameAnti-ARRDC4 antibody
See all ARRDC4 primary antibodies
DescriptionRabbit polyclonal to ARRDC4
Tested applicationsSuitable for: WB, Indirect ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from an internal region of human ARRDC4.
- extracts from COLO cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).|
|ICC/IF||Use at an assay dependent concentration. PubMed: 23236378|
Sequence similaritiesBelongs to the arrestin family.
- Information by UniProt
- ARRD4_HUMAN antibody
- ARRDC 4 antibody
- Arrdc4 antibody
All lanes : Anti-ARRDC4 antibody (ab74265) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from COLO cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Shea FF et al. Mammalian alpha arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with beta arrestins. PLoS One 7:e50557 (2012). ICC/IF ; Human . Read more (PubMed: 23236378) »