Key features and details
- Rabbit polyclonal to ARRDC4
- Suitable for: WB, Indirect ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARRDC4 antibody
DescriptionRabbit polyclonal to ARRDC4
Tested applicationsSuitable for: WB, Indirect ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from an internal region of human ARRDC4.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).|
Sequence similaritiesBelongs to the arrestin family.
- Information by UniProt
- ARRD4_HUMAN antibody
- ARRDC 4 antibody
- Arrdc4 antibody
All lanes : Anti-ARRDC4 antibody (ab74265) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from COLO cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
ab74265 has been referenced in 1 publication.
- Shea FF et al. Mammalian alpha arrestins link activated seven transmembrane receptors to Nedd4 family e3 ubiquitin ligases and interact with beta arrestins. PLoS One 7:e50557 (2012). ICC/IF ; Human . PubMed: 23236378