Overview

  • Product name
    Anti-Arrestin 3 antibody [ZPR-1]
  • Description
    Mouse monoclonal [ZPR-1] to Arrestin 3
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Frmore details
  • Species reactivity
    Reacts with: Zebrafish
  • Immunogen

    Tissue, cells or virus corresponding to Zebrafish Arrestin 3. Whole adult retinal cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • Purity
    SDS-PAGE
  • Purification notes
    ab174435 is produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. Near homogeneity as judged by SDS-PAGE.
  • Clonality
    Monoclonal
  • Clone number
    ZPR-1
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500.

Target

  • Relevance
    Vision of high temporal resolution depends on careful regulation of photoresponse kinetics, beginning with the lifetime of activated photopigment. The activity of rhodopsin is quenched by high-affinity binding of arrestin to photoexcited phosphorylated photopigment, which effectively terminates the visual transduction cascade. subfunctionalized expression of Arr3a is in M- and L-cones, and Arr3b is in S- and UV-cones. The inactivation of arr3a by morpholino knockdown technology resulted in a severe delay in photoresponse recovery which, under bright light conditions, was rate-limiting. Arr3a activity partially overlapped with the function of the cone-specific kinase Grk7a involved in initial response recovery. Tissue specificity : Retinal cone cells, retinal outer nuclear layer, retinal photoreceptor layer, ciliary marginal zone, epiphysis, outer limiting membrane.
  • Database links
  • Alternative names
    • arr3a antibody
    • arr3l antibody
    • Arrestin 3, retinal (X-arrestin), like antibody
    • c-arrestin antibody
    • zgc:92697 antibody
    see all

Images

  • ab174435 staining arrestin in 4 dpf zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab174435 at 1/500 in blocking solution for 16h at 4°C. An alexa 568 conjugated goat anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Arrestin is present in photoreceptors (red)

    See Abreview

References

This product has been referenced in:
  • Huang DF  et al. Zebrafish Lacking Circadian Gene per2 Exhibit Visual Function Deficiency. Front Behav Neurosci 12:53 (2018). IHC-P ; Zebrafish . Read more (PubMed: 29593513) »
  • Albalawi A  et al. Carnosic acid attenuates acrylamide-induced retinal toxicity in zebrafish embryos. Exp Eye Res 175:103-114 (2018). Read more (PubMed: 29928899) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Mouse retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 19 2016

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Zebrafish retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Zebrafish retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 19 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Retina, Photoreceptors)
Specification
Retina, Photoreceptors
Permeabilization
No
Fixative
Paraformaldehyde

Dr. Ryan Macdonald

Verified customer

Submitted Aug 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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