Anti-ARS2 antibody - C-terminal (ab220991)
Key features and details
- Rabbit polyclonal to ARS2 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARS2 antibody - C-terminal
See all ARS2 primary antibodies -
Description
Rabbit polyclonal to ARS2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Chinese hamster, Orangutan -
Immunogen
Recombinant fragment corresponding to Human ARS2 aa 750 to the C-terminus (C terminal).
Database link: Q9BXP5 -
Positive control
- RT-4 and U-251 MG cell lysates; MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 101 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 101 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and DROSHA, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the ARS2 family. -
Cellular localization
Nucleus > nucleoplasm. Cytoplasm. Predominantly nuclear. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent way. - Information by UniProt
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Database links
- Entrez Gene: 100689205 Chinese hamster
- Entrez Gene: 527938 Cow
- Entrez Gene: 51593 Human
- Entrez Gene: 83701 Mouse
- Entrez Gene: 686980 Rat
- Omim: 614469 Human
- SwissProt: Q60436 Chinese hamster
- SwissProt: A4IFB1 Cow
see all -
Alternative names
- ARS2 protein antibody
- Arsenate resistance protein ARS2 antibody
- Arsenite resistance protein 2 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab220991 has been referenced in 1 publication.
- Iwasaki M et al. Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells. iScience 25:104289 (2022). PubMed: 35573189