Recombinant Anti-ARSA/ASA antibody [EPR11039] (ab174844)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11039] to ARSA/ASA
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ARSA/ASA antibody [EPR11039]
See all ARSA/ASA primary antibodies -
Description
Rabbit monoclonal [EPR11039] to ARSA/ASA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: MouseDoes not react with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skin, A431 and HeLa lysates; Human colon and kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11039 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/1000 - 1/5000. Detects a band of approximately 60-62 kDa (predicted molecular weight: 54 kDa).
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000 - 1/5000. Detects a band of approximately 60-62 kDa (predicted molecular weight: 54 kDa). |
Target
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Function
Hydrolyzes cerebroside sulfate. -
Involvement in disease
Defects in ARSA are a cause of leukodystrophy metachromatic (MLD) [MIM:250100]. MLD is a disease due to a lysosomal storage defect. It is characterized by intralysosomal storage of cerebroside-3-sulfate in neural and non-neural tissues, with a diffuse loss of myelin in the central nervous system. Progressive demyelination causes a variety of neurological symptoms, including gait disturbances, ataxias, optical atrophy, dementia, seizures, and spastic tetraparesis. Three forms of the disease can be distinguished according to the age at onset: late-infantile, juvenile and adult.
Arylsulfatase A activity is defective in multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a disorder characterized by decreased activity of all known sulfatases. MSD is due to defects in SUMF1 resulting in the lack of post-translational modification of a highly conserved cysteine into 3-oxoalanine. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. -
Sequence similarities
Belongs to the sulfatase family. -
Post-translational
modificationsThe conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD). -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 410 Human
- Entrez Gene: 11883 Mouse
- Omim: 607574 Human
- SwissProt: P15289 Human
- SwissProt: P50428 Mouse
- Unigene: 88251 Human
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Alternative names
- arsA antibody
- ARSA_HUMAN antibody
- arylsulfatase A antibody
see all
Images
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All lanes : Anti-ARSA/ASA antibody [EPR11039] (ab174844) at 1/1000 dilution
Lane 1 : Human skin lysates
Lane 2 : A431 cell lysates
Lane 3 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling ARSA/ASA with ab174844 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling ARSA/ASA with ab174844 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab174844 has been referenced in 1 publication.
- Yoo HS et al. Changes in plasma arylsulfatase A level as a compensatory biomarker of early Parkinson's disease. Sci Rep 10:5567 (2020). PubMed: 32221382