Anti-ARSA/ASA antibody (ab77586)
Key features and details
- Goat polyclonal to ARSA/ASA
- Suitable for: IHC-P, WB, ICC, Flow Cyt (Intra)
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ARSA/ASA antibody
See all ARSA/ASA primary antibodies -
Description
Goat polyclonal to ARSA/ASA -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WB, ICC, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey -
Immunogen
Synthetic peptide corresponding to Human ARSA/ASA aa 429-440 (internal sequence).
Sequence:C-YDLSKDPGENYN
Database link: NP_000478.2 -
Positive control
- IHC: Human cortex staining WB: Mouse testis lysates and Recombinant Human ARSA/ASA protein (ab116931) Flow Cyt (intra): HeLa cells ICC: HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab77586 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
1 hour primary incubation is recommended for this product. Approx 60-65Da band observed in Mouse and Rat Testis lysates. |
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ICC |
Use a concentration of 10 µg/ml.
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Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). 1 hour primary incubation is recommended for this product. Approx 60-65Da band observed in Mouse and Rat Testis lysates. |
ICC
Use a concentration of 10 µg/ml. |
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
Target
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Function
Hydrolyzes cerebroside sulfate. -
Involvement in disease
Defects in ARSA are a cause of leukodystrophy metachromatic (MLD) [MIM:250100]. MLD is a disease due to a lysosomal storage defect. It is characterized by intralysosomal storage of cerebroside-3-sulfate in neural and non-neural tissues, with a diffuse loss of myelin in the central nervous system. Progressive demyelination causes a variety of neurological symptoms, including gait disturbances, ataxias, optical atrophy, dementia, seizures, and spastic tetraparesis. Three forms of the disease can be distinguished according to the age at onset: late-infantile, juvenile and adult.
Arylsulfatase A activity is defective in multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a disorder characterized by decreased activity of all known sulfatases. MSD is due to defects in SUMF1 resulting in the lack of post-translational modification of a highly conserved cysteine into 3-oxoalanine. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. -
Sequence similarities
Belongs to the sulfatase family. -
Post-translational
modificationsThe conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD). -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 410 Human
- Entrez Gene: 11883 Mouse
- Omim: 607574 Human
- SwissProt: P15289 Human
- SwissProt: P50428 Mouse
- Unigene: 88251 Human
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Alternative names
- arsA antibody
- ARSA_HUMAN antibody
- arylsulfatase A antibody
see all
Images
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Immunofluorescence analysis of HeLa cells labelling ARSA/ASA with ab77586 at 10 µg/mL. Cells were permeabilized with 0.15% Triton X-100. Alexa Fluor 488 secondary antibody (2ug/ml). Nuclear DNA was labelled with DAPI (blue). Negative control: Unimmunized goat IgG 10 µg/mL.
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Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling ARSA/ASA with ab77586 at 10 µg/mL. Cells were permeabilised with 0.5% Triton. Alexa Fluor 488 secondary antibody (1ug/ml). Unimmunized goat IgG was used as the isotype control (black).
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Anti-ARSA/ASA antibody (ab77586) at 0.5 µg/ml + Mouse Testis lysate (in RIPA buffer) at 35 µg
Predicted band size: 54 kDa
Observed band size: 54 kDa
Primary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (0)
ab77586 has not yet been referenced specifically in any publications.