Product nameAnti-ARSA/ASA antibody
See all ARSA/ASA primary antibodies
DescriptionRabbit polyclonal to ARSA/ASA
Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human ARSA/ASA (internal sequence).
- Human cervix carcinoma tissue. Jurkat and COLO 205 cell extracts.
Previously labelled as ARSA.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/1000. Predicted molecular weight: 54 kDa.|
FunctionHydrolyzes cerebroside sulfate.
Involvement in diseaseDefects in ARSA are a cause of leukodystrophy metachromatic (MLD) [MIM:250100]. MLD is a disease due to a lysosomal storage defect. It is characterized by intralysosomal storage of cerebroside-3-sulfate in neural and non-neural tissues, with a diffuse loss of myelin in the central nervous system. Progressive demyelination causes a variety of neurological symptoms, including gait disturbances, ataxias, optical atrophy, dementia, seizures, and spastic tetraparesis. Three forms of the disease can be distinguished according to the age at onset: late-infantile, juvenile and adult.
Arylsulfatase A activity is defective in multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a disorder characterized by decreased activity of all known sulfatases. MSD is due to defects in SUMF1 resulting in the lack of post-translational modification of a highly conserved cysteine into 3-oxoalanine. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay.
Sequence similaritiesBelongs to the sulfatase family.
modificationsThe conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD).
- Information by UniProt
- arsA antibody
- ARSA_HUMAN antibody
- arylsulfatase A antibody
ab74804 at 1/50 dilution staining ARSA/ASA in human cervix carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
All lanes : Anti-ARSA/ASA antibody (ab74804) at 1/500 dilution
Lane 1 : Jurkat cell extracts
Lane 2 : COLO 205 cell extracts
Lane 3 : Jurkat cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
ab74804 has not yet been referenced specifically in any publications.