• Product name

  • Description

    Goat polyclonal to ARSA/ASA
  • Host species

  • Tested applications

    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Human, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human ARSA/ASA aa 429-440 (internal sequence).


    Database link: NP_000478.2

  • Positive control

    • Recombinant Human ARSA/ASA protein (ab116931) can be used as a positive control in WB. Mouse testis lysates.
  • General notes

    Previously labelled as ARSA. 



Our Abpromise guarantee covers the use of ab77586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ELISA Use at an assay dependent concentration.


  • Function

    Hydrolyzes cerebroside sulfate.
  • Involvement in disease

    Defects in ARSA are a cause of leukodystrophy metachromatic (MLD) [MIM:250100]. MLD is a disease due to a lysosomal storage defect. It is characterized by intralysosomal storage of cerebroside-3-sulfate in neural and non-neural tissues, with a diffuse loss of myelin in the central nervous system. Progressive demyelination causes a variety of neurological symptoms, including gait disturbances, ataxias, optical atrophy, dementia, seizures, and spastic tetraparesis. Three forms of the disease can be distinguished according to the age at onset: late-infantile, juvenile and adult.
    Arylsulfatase A activity is defective in multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a disorder characterized by decreased activity of all known sulfatases. MSD is due to defects in SUMF1 resulting in the lack of post-translational modification of a highly conserved cysteine into 3-oxoalanine. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay.
  • Sequence similarities

    Belongs to the sulfatase family.
  • Post-translational

    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD).
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • arsA antibody
    • ARSA_HUMAN antibody
    • arylsulfatase A antibody
    • Arylsulfatase A component C antibody
    • As 2 antibody
    • AS A antibody
    • As2 antibody
    • ASA antibody
    • AW212749 antibody
    • C230037L18Rik antibody
    • Cerebroside-sulfatase antibody
    • metachromatic leucodystrophy antibody
    • MGC125207 antibody
    • MLD antibody
    • OTTHUMP00000196546 antibody
    • OTTHUMP00000196548 antibody
    • TISP73 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human Cortex staining ARSA/ASA with ab77586at 5 μg/ml

  • Anti-ARSA/ASA antibody (ab77586) at 0.5 µg/ml + Mouse Testis lysate (in RIPA buffer) at 35 µg

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

    Primary incubation was 1 hour.


ab77586 has not yet been referenced specifically in any publications.

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