Key features and details
- Goat polyclonal to ARSA/ASA
- Suitable for: Flow Cyt, IHC-P, WB, ELISA
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ARSA/ASA antibody
See all ARSA/ASA primary antibodies
DescriptionGoat polyclonal to ARSA/ASA
Tested applicationsSuitable for: Flow Cyt, IHC-P, WB, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee, Rhesus monkey
- IHC: Human cortex staining WB: Mouse testis lysates and Recombinant Human ARSA/ASA protein (ab116931 Flow Cyt: HeLa cells IF: HeLa cells
Previously labelled as ARSA.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab77586 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab77586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IF||Use a concentration of 10 µg/ml.|
|Flow Cyt||Use a concentration of 10 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
1 hour primary incubation is recommended for this product.
Approx 60-65Da band observed in Mouse and Rat Testis lysates.
FunctionHydrolyzes cerebroside sulfate.
Involvement in diseaseDefects in ARSA are a cause of leukodystrophy metachromatic (MLD) [MIM:250100]. MLD is a disease due to a lysosomal storage defect. It is characterized by intralysosomal storage of cerebroside-3-sulfate in neural and non-neural tissues, with a diffuse loss of myelin in the central nervous system. Progressive demyelination causes a variety of neurological symptoms, including gait disturbances, ataxias, optical atrophy, dementia, seizures, and spastic tetraparesis. Three forms of the disease can be distinguished according to the age at onset: late-infantile, juvenile and adult.
Arylsulfatase A activity is defective in multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a disorder characterized by decreased activity of all known sulfatases. MSD is due to defects in SUMF1 resulting in the lack of post-translational modification of a highly conserved cysteine into 3-oxoalanine. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay.
Sequence similaritiesBelongs to the sulfatase family.
modificationsThe conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD).
- Information by UniProt
- arsA antibody
- ARSA_HUMAN antibody
- arylsulfatase A antibody
Immunofluorescence analysis of HeLa cells labelling ARSA/ASA with ab77586 at 10 µg/mL. Cells were permeabilized with 0.15% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 2 µg/mL was used as the secondary antibody (green). Nuclear DNA was labelled with DAPI (blue). Negative control: Unimmunized goat IgG 10 µg/mL.
Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling ARSA/ASA with ab77586 at 10 µg/mL. Cells were permeabilised with 0.5% Triton. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1 µg/mL was used as the secondary antibody. Unimmunized goat IgG was used as the isotype control (black).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human Cortex staining ARSA/ASA with ab77586at 5 μg/ml
Anti-ARSA/ASA antibody (ab77586) at 0.5 µg/ml + Mouse Testis lysate (in RIPA buffer) at 35 µg
Predicted band size: 54 kDa
Observed band size: 54 kDa
Primary incubation was 1 hour.
ab77586 has not yet been referenced specifically in any publications.