Anti-ARSB antibody (ab85727)
Key features and details
- Goat polyclonal to ARSB
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARSB antibody
See all ARSB primary antibodies -
Description
Goat polyclonal to ARSB -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Cat -
Immunogen
Synthetic peptide corresponding to Human ARSB (internal sequence) (Cysteine residue).
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Positive control
- Skeletal muscle tissue; human heart lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 60 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 60 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Involvement in disease
Defects in ARSB are the cause of mucopolysaccharidosis type 6 (MPS6) [MIM:253200]; also known as Maroteaux-Lamy syndrome. MPS6 is an autosomal recessive lysosomal storage disease characterized by intracellular accumulation of dermatan sulfate. Clinical features can include abnormal growth, short stature, stiff joints, skeletal malformations, corneal clouding, hepatosplenomegaly, and cardiac abnormalities. A wide variation in clinical severity is observed.
Arylsulfatase B activity is defective in multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a disorder characterized by decreased activity of all known sulfatases. MSD is due to defects in SUMF1 resulting in the lack of post-translational modification of a highly conserved cysteine into 3-oxoalanine. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. -
Sequence similarities
Belongs to the sulfatase family. -
Post-translational
modificationsThe conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD). -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 100216331 Cat
- Entrez Gene: 411 Human
- Omim: 611542 Human
- SwissProt: P33727 Cat
- SwissProt: P15848 Human
- Unigene: 149103 Human
- Unigene: 604199 Human
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Alternative names
- Arsb antibody
- ARSB_HUMAN antibody
- Arylsulfatase B antibody
see all
Images
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ab85727, at 5 µg/ml, staining ARSB in skeletal muscle tissue by Immunohistochemistry.
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Anti-ARSB antibody (ab85727) at 0.03 µg/ml + human heart lysate at 35 µg
Predicted band size: 60 kDa
Observed band size: 35,40 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab85727 has been referenced in 1 publication.
- Luo M et al. Changes in the metabolism of chondroitin sulfate glycosaminoglycans in articular cartilage from patients with Kashin-Beck disease. Osteoarthritis Cartilage 22:986-95 (2014). PubMed: 24857976