Overview

  • Product name

    Anti-ARSD antibody
  • Description

    Goat polyclonal to ARSD
  • Host species

    Goat
  • Specificity

    ab81978 is expected to recognise both reported isoforms (NP_001660.2; NP_033667.2).
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ARSD aa 108-120 (internal sequence). (NP_001660.2; NP_033667.2)
    Sequence:

    C-DASNGYRALQWNA

  • Positive control

    • A459 and HepG2 cell lysates.
  • General notes

    Previously labelled as Arylsulfatase D. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 0.05% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab81978 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/16,000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificity

      Expressed in the pancreas, kidney, liver, lung, placenta, brain and heart.
    • Sequence similarities

      Belongs to the sulfatase family.
    • Post-translational
      modifications

      The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
    • Cellular localization

      Lysosome.
    • Information by UniProt
    • Database links

    • Alternative names

      • arsD antibody
      • ARSD_HUMAN antibody
      • Arylsulfatase D antibody
      • ASD antibody
      • EC 3.1.6 antibody
      • OTTHUMP00000022848 antibody
      • OTTHUMP00000022849 antibody
      see all

    Images

    • All lanes : Anti-ARSD antibody (ab81978) at 0.3 µg/ml

      Lane 1 : A459 lysate in RIPA buffer
      Lane 2 : A459 lysate in RIPA buffer with immunising peptide

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 65 kDa
      Observed band size: 65 kDa
      Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.



      An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide.
      Primary incubation was 1 hour. Detected by chemiluminescence.

    References

    ab81978 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab81978.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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