Overview

  • Product name

  • Description

    Rabbit polyclonal to ART1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide selected from the central region of human ART1 conjugated to KLH.

  • Positive control

    • HepG2 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab71295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

Target

  • Relevance

    ART1 belongs to the Arg-specific ADP-ribosyltransferase family. It catalyzes the transfer of ADP-ribose from NAD+ to arginine residues in proteins.
  • Cellular localization

    Sarcoplasmic reticulum membrane; Lipid-anchor, GPI-anchor.
  • Database links

  • Alternative names

    • ADP ribosyltransferase 1 antibody
    • ADP ribosyltransferase 2 antibody
    • ART 1 antibody
    • ART2 antibody
    • CD296 antibody
    • CD296 antigen antibody
    • GPI linked NAD(P)(+) arginine ADP ribosyltransferase 1 antibody
    • MGC133217 antibody
    • Mono(ADP ribosyl)transferase antibody
    • RT6 antibody
    see all

Images

  • Anti-ART1 antibody (ab71295) at 1/13 dilution + HepG2 cell line lysate at 35 µg

    Predicted band size: 36 kDa
    Observed band size: 36 kDa
    Additional bands at: 24 kDa, 30 kDa, 40 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Fabrizio G  et al. ARTC1-mediated ADP-ribosylation of GRP78/BiP: a new player in endoplasmic-reticulum stress responses. Cell Mol Life Sci 72:1209-25 (2015). WB ; Human . Read more (PubMed: 25292337) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - saponin
Fixative
Methanol

Miss. Gaia Fabrizio

Verified customer

Submitted Oct 24 2014

Answer

Thank you for contacting us.

Here is the link to the database entry I was refering to
http://www.uniprot.org/uniprot/P52961

There you will find some information about the disulfide bonds as well as the glycosylation sites.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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