The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/312500.
ART4 is a member of the ADP ribosyltransferase gene family. Antigens of the Dombrock blood group system are located on the gene product, which is glycosylphosphatidylinosotol anchored to the erythrocyte membrane. Allelic variants, some of which lead to adverse transfusion reactions, are known.