Overview

  • Product name

    Anti-ART4/DOK1 antibody
  • Description

    Rabbit polyclonal to ART4/DOK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep, Rabbit, Horse, Cow, Pig, Chimpanzee, African green monkey
  • Immunogen

    Synthetic peptide corresponding to Human ART4/DOK1 aa 179-228 (internal sequence).
    Database link: NP_066549

  • Positive control

    • Human Fetal heart lysate.
  • General notes

     This product was previously labelled as ART4

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

Target

  • Tissue specificity

    Expressed in spleen and T-cells.
  • Sequence similarities

    Belongs to the Arg-specific ADP-ribosyltransferase family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP ribosyltransferase 4 (DO blood group) antibody
    • ADP ribosyltransferase 4 (Dombrock blood group) antibody
    • ADP ribosyltransferase 4 antibody
    • ADP-ribosyltransferase C2 and C3 toxin-like 4 antibody
    • ART 4 antibody
    • Art4 antibody
    • ARTC4 antibody
    • CD297 antibody
    • CD297 antigen antibody
    • DO antibody
    • DOK1 antibody
    • Dombrock blood group carrier molecule antibody
    • Ecto ADP ribosyltransferase 4 antibody
    • Ecto-ADP-ribosyltransferase 4 antibody
    • Mono ADP ribosyltransferase 4 antibody
    • Mono(ADP ribosyl)transferase 4 antibody
    • Mono(ADP-ribosyl)transferase 4 antibody
    • NAD(P)(+) arginine ADP ribosyltransferase 4 antibody
    • NAD(P)(+)--arginine ADP-ribosyltransferase 4 antibody
    • NAR4_HUMAN antibody
    see all

Images

  • Anti-ART4/DOK1 antibody (ab83342) at 1/1 dilution + fetal heart lysate at 10 µg/ml

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Gel concentration 12%

References

ab83342 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - 0.2% Triton X-100
Specification
Heart
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 26 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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