Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-ART4 antibody

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - 0.2% Triton X-100
Specification
Heart
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.05% Serum + 0.01% Triton X-100 in PBS
Dilution
1/500

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: Anti-Rabbit 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

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Submitted Oct 26 2016

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