Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - 0.2% Triton X-100
Specification
Heart
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.05% Serum + 0.01% Triton X-100 in PBS
Dilution
1/500
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Anti-Rabbit 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Abcam user community
Verified customer
Submitted Oct 26 2016