Key features and details
- Rabbit polyclonal to ARTD10
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ARTD10 antibody
DescriptionRabbit polyclonal to ARTD10
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human ARTD10 aa 300-350. NP_116178.1
- WB: Whole cell lysate from HeLa cells.
Protein previously known as PARP10.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/2500. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).|
|IP||Use at 1-4 µg/mg of lysate.|
RelevancePoly (ADP ribose) polymerases (PARPs), such as PARP10, regulate gene transcription by altering chromatin organization by adding ADP ribose to histones. PARPs can also function as transcriptional cofactors
- ADP ribosyltransferase diphtheria toxin like 10 antibody
- ARTD10 antibody
- PARP 10 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labeling ARTD10 with ab70800 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-ARTD10 antibody (ab70800) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 123 kDa
Observed band size: 123 kDa
Additional bands at: 60 kDa, 70 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
1mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70800 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of immunoprecipitate was loaded per lane, and probed with ab70800 at 1ug/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab70800 has not yet been referenced specifically in any publications.