Synthetic peptide within Human ARTD10 aa 300-350. The exact sequence is proprietary. NP_116178.1
Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Laryngeal Squamous Cell Carcinoma, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Small Cell Lung Cancer
Mouse control: Squamous Cell Carcinoma
Protein previously known as PARP10.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide Constituents: 0.1% BSA, Tris buffered saline
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Immunogen affinity purified
ab84917 was affinity purified using an epitope specific to ARTD10 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Poly (ADP ribose) polymerases (PARPs), such as PARP10, regulate gene transcription by altering chromatin organization by adding ADP ribose to histones. PARPs can also function as transcriptional cofactors