Recombinant Anti-Artemin antibody [EPR9763] - BSA and Azide free (ab250054)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9763] to Artemin - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Artemin antibody [EPR9763] - BSA and Azide free
See all Artemin primary antibodies -
Description
Rabbit monoclonal [EPR9763] to Artemin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: T-47D, NIH/3T3 and PC-12 whole cell lysate. Mouse and rat brain lysate; PC-12 whole cell lysate. IP: PC-12 whole cell lysate
-
General notes
ab250054 is the carrier-free version of ab178434.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9763 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
|
|
IP |
Use at an assay dependent concentration.
|
Notes |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 23 kDa. |
IP
Use at an assay dependent concentration. |
Target
-
Function
Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. -
Tissue specificity
Ubiquitous. Expressed at high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. Expressed at low levels in the brain. -
Sequence similarities
Belongs to the TGF-beta family. GDNF subfamily. -
Developmental stage
Expressed during embryogenesis. High level expression seen in fetal kidney and lung while a low level expression seen in the fetal brain. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 9048 Human
- Entrez Gene: 11876 Mouse
- Entrez Gene: 362572 Rat
- Omim: 603886 Human
- SwissProt: Q5T4W7 Human
- SwissProt: Q9Z0L2 Mouse
- SwissProt: Q6AYE8 Rat
- Unigene: 632404 Human
see all -
Alternative names
- Artemin antibody
- Artn antibody
- ARTN_HUMAN antibody
see all
Images
-
This data was developed using ab178434, the same antibody clone in a different buffer formulation.
Purified ab178434 at 1/30 dilution (2µg) immunoprecipitating Artemin in PC-12 whole cell lysate.
Lane 1 (input): PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate 10µg
Lane 2 (+): ab178434 + PC-12 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab178434 in PC-12 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 23 kDa -
All lanes : Anti-Artemin antibody [EPR9763] (ab178434) at 1/1000 dilution
Lane 1 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : Rat brain lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 23 kDaThis data was developed using ab178434, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab250054 has not yet been referenced specifically in any publications.