Overview

  • Product name

    Anti-Artemin antibody [IF3]
    See all Artemin primary antibodies
  • Description

    Mouse monoclonal [IF3] to Artemin
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Artemin. Recombinant human Artemin protein produced using CHO based proprietary suspension cell line. Immunogen is purified from cell culture supernatant.
    Database link: Q5T4W7

  • Positive control

    • U2OS cells transfected with GFRa3-Ret51 expression vector and treated with Artemin. FFPE human cerebral cortex tissue sections from Alzheimer's disease patients.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    IgG fraction purified with Protein G affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    IF3
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 0.3 - 20 µg/ml.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain.
    • Tissue specificity

      Ubiquitous. Expressed at high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. Expressed at low levels in the brain.
    • Sequence similarities

      Belongs to the TGF-beta family. GDNF subfamily.
    • Developmental stage

      Expressed during embryogenesis. High level expression seen in fetal kidney and lung while a low level expression seen in the fetal brain.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Artemin antibody
      • Artn antibody
      • ARTN_HUMAN antibody
      • Enovin antibody
      • EVN antibody
      • NBN antibody
      • Neublastin antibody
      • Neurotrophic factor antibody
      see all

    Images

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cerebral cortex tissue sections from Alzheimer's disease patients labeling Artemin with ab205564 at 5 µg/mL. DAB was used for visualization. Sections were counterstained with toluidine blue.

    • Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin. Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected with ab205564 at 20 µg/mL. Goat anti-mouse Dylight 488 conjugated antibody (1 µg/mL) was used as secondary antibody. For nuclear staining DAPI was used. Left image; Artemin bound to the GFRa3-Ret51 receptor complex; Right image; Negative control - GFRa3-Ret51 receptor expressing U2OS cells.

       

       

    References

    ab205564 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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