Key features and details
- Mouse monoclonal [IF3] to Artemin
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Artemin antibody [IF3]
See all Artemin primary antibodies
DescriptionMouse monoclonal [IF3] to Artemin
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Artemin. Recombinant human Artemin protein produced using CHO based proprietary suspension cell line. Immunogen is purified from cell culture supernatant.
Database link: Q5T4W7
- U2OS cells transfected with GFRa3-Ret51 expression vector and treated with Artemin. FFPE human cerebral cortex tissue sections from Alzheimer's disease patients.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesIgG fraction purified with Protein G affinity chromatography.
Our Abpromise guarantee covers the use of ab205564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.3 - 20 µg/ml.|
FunctionLigand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain.
Tissue specificityUbiquitous. Expressed at high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. Expressed at low levels in the brain.
Sequence similaritiesBelongs to the TGF-beta family. GDNF subfamily.
Developmental stageExpressed during embryogenesis. High level expression seen in fetal kidney and lung while a low level expression seen in the fetal brain.
- Information by UniProt
- Artemin antibody
- Artn antibody
- ARTN_HUMAN antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cerebral cortex tissue sections from Alzheimer's disease patients labeling Artemin with ab205564 at 5 µg/mL. DAB was used for visualization. Sections were counterstained with toluidine blue.
Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin. Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected with ab205564 at 20 µg/mL. Goat anti-mouse Dylight 488 conjugated antibody (1 µg/mL) was used as secondary antibody. For nuclear staining DAPI was used. Left image; Artemin bound to the GFRa3-Ret51 receptor complex; Right image; Negative control - GFRa3-Ret51 receptor expressing U2OS cells.
ab205564 has not yet been referenced specifically in any publications.