Anti-ARTS1/ERAP1 antibody - C-terminal (ab181390)
Key features and details
- Rabbit polyclonal to ARTS1/ERAP1 - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-ARTS1/ERAP1 antibody - C-terminal
See all ARTS1/ERAP1 primary antibodies -
Description
Rabbit polyclonal to ARTS1/ERAP1 - C-terminal -
Host species
Rabbit -
Specificity
At least two isoforms of ARTS1/ERAP1 are known to exist; ab181390 will detect both isoforms. ab181390 is predicted to not cross-react with ERAP2.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human ARTS1/ERAP1 (C terminal). The exact sequence is proprietary. 19 amino acids.
Database link: Q9NZ08 -
Positive control
- SK-N-SH cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 107 kDa).
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 107 kDa). |
Target
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Function
Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the peptidase M1 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 51752 Human
- Omim: 606832 Human
- SwissProt: Q9NZ08 Human
- Unigene: 716426 Human
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Alternative names
- A LAP antibody
- A-LAP antibody
- Adipocyte derived leucine aminopeptidase antibody
see all
Images
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Lane 1 : Anti-ARTS1/ERAP1 antibody - C-terminal (ab181390) at 1 µg/ml
Lane 2 : Anti-ARTS1/ERAP1 antibody - C-terminal (ab181390) at 2 µg/ml
Lane 1 : SK-N-SH (Human neuroblastoma cell line) whole cell lysate
Lane 2 : SK-N-SH whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 107 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab181390 has not yet been referenced specifically in any publications.