Key features and details
- Rabbit polyclonal to ARTS1/ERAP1 - C-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ARTS1/ERAP1 antibody - C-terminal
See all ARTS1/ERAP1 primary antibodies
DescriptionRabbit polyclonal to ARTS1/ERAP1 - C-terminal
At least two isoforms of ARTS1/ERAP1 are known to exist; ab181390 will detect both isoforms. ab181390 is predicted to not cross-react with ERAP2.
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human ARTS1/ERAP1 (C terminal). The exact sequence is proprietary. 19 amino acids.
Database link: Q9NZ08
- SK-N-SH cell lysate. Mouse brain tissue.
This product was previously labelled as ARTS1
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab181390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 107 kDa).|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionAminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.
Sequence similaritiesBelongs to the peptidase M1 family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- A LAP antibody
- A-LAP antibody
- Adipocyte derived leucine aminopeptidase antibody
Lane 1 : Anti-ARTS1/ERAP1 antibody - C-terminal (ab181390) at 1 µg/ml
Lane 2 : Anti-ARTS1/ERAP1 antibody - C-terminal (ab181390) at 2 µg/ml
All lanes : SK-N-SH whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 107 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of mouse brain tissue, staining ARTS1/ERAP1 with ab181390 at 5 µg/ml.
Immunofluorescence of mouse brain tissue, staining ARTS1/ERAP1 with ab181390 at 20 μg/mL.
ab181390 has not yet been referenced specifically in any publications.