Overview

  • Product name

    Anti-ARTS1/ERAP1 antibody - C-terminal
    See all ARTS1/ERAP1 primary antibodies
  • Description

    Rabbit polyclonal to ARTS1/ERAP1 - C-terminal
  • Host species

    Rabbit
  • Specificity

    At least two isoforms of ARTS1/ERAP1 are known to exist; ab181390 will detect both isoforms. ab181390 is predicted to not cross-react with ERAP2.

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ARTS1/ERAP1 (C terminal). The exact sequence is proprietary. 19 amino acids.
    Database link: Q9NZ08

  • Positive control

    • SK-N-SH cell lysate. Mouse brain tissue.
  • General notes

     This product was previously labelled as ARTS1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab181390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 107 kDa).
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function

    Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the peptidase M1 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A LAP antibody
    • A-LAP antibody
    • Adipocyte derived leucine aminopeptidase antibody
    • Adipocyte-derived leucine aminopeptidase antibody
    • ALAP antibody
    • Aminopeptidase PILS antibody
    • Aminopeptidase regulator of TNFR1 shedding antibody
    • APPILS antibody
    • ARTS 1 antibody
    • ARTS-1 antibody
    • Arts1 antibody
    • Endoplasmic reticulum aminopeptidase 1 antibody
    • Endoplasmic reticulum aminopeptidase associated with antigen processing antibody
    • ERAAP antibody
    • ERAAP1 antibody
    • ERAP 1 antibody
    • Erap1 antibody
    • ERAP1_HUMAN antibody
    • KIAA0525 antibody
    • kiaa0525 protein antibody
    • PILS AP antibody
    • PILS-AP antibody
    • PILSA antibody
    • PILSAP antibody
    • Puromycin insensitive leucyl specific aminopeptidase antibody
    • Puromycin-insensitive leucyl-specific aminopeptidase antibody
    • Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator antibody
    • type 1 tumor necrosis factor receptors shedding aminopeptidase regulator antibody
    • VEGF-induced aminopeptidase antibody
    see all

Images

  • Lane 1 : Anti-ARTS1/ERAP1 antibody - C-terminal (ab181390) at 1 µg/ml
    Lane 2 : Anti-ARTS1/ERAP1 antibody - C-terminal (ab181390) at 2 µg/ml

    All lanes : SK-N-SH whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 107 kDa
    Observed band size: 105 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of mouse brain tissue, staining ARTS1/ERAP1 with ab181390 at 5 µg/ml.

  • Immunofluorescence of mouse brain tissue, staining ARTS1/ERAP1 with ab181390 at 20 μg/mL.

References

ab181390 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab181390.
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