RecombinantRabMAb

Recombinant Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

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Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
  • Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
  • Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
  • Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6069] to ARTS1/ERAP1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ARTS1/ERAP1 antibody [EPR6069]
    See all ARTS1/ERAP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6069] to ARTS1/ERAP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human ARTS1/ERAP1 aa 900-1000 (C terminal).

  • Positive control

    • WB: K562, HUVEC, mouse heart, rat spleen and A431 cell lysates. IHC-P: Human uterus and human bladder carcinoma tissue. Flow Cyt: K562 cells.

  • General notes

     This product was previously labelled as ARTS1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 107 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/50 - 1/100.

 
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 107 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/50 - 1/100.

 
  • Application notes
    Is unsuitable for Flow Cyt or ICC.

    • Target

    • Function

      Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.

    • Tissue specificity

      Ubiquitous.

    • Sequence similarities

      Belongs to the peptidase M1 family.

    • Post-translational
      modifications

      N-glycosylated.

    • Cellular localization

      Endoplasmic reticulum membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • A LAP antibody
      • A-LAP antibody
      • Adipocyte derived leucine aminopeptidase antibody
      • Adipocyte-derived leucine aminopeptidase antibody
      • ALAP antibody
      • Aminopeptidase PILS antibody
      • Aminopeptidase regulator of TNFR1 shedding antibody
      • APPILS antibody
      • ARTS 1 antibody
      • ARTS-1 antibody
      • Arts1 antibody
      • Endoplasmic reticulum aminopeptidase 1 antibody
      • Endoplasmic reticulum aminopeptidase associated with antigen processing antibody
      • ERAAP antibody
      • ERAAP1 antibody
      • ERAP 1 antibody
      • Erap1 antibody
      • ERAP1_HUMAN antibody
      • KIAA0525 antibody
      • kiaa0525 protein antibody
      • PILS AP antibody
      • PILS-AP antibody
      • PILSA antibody
      • PILSAP antibody
      • Puromycin insensitive leucyl specific aminopeptidase antibody
      • Puromycin-insensitive leucyl-specific aminopeptidase antibody
      • Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator antibody
      • type 1 tumor necrosis factor receptors shedding aminopeptidase regulator antibody
      • VEGF-induced aminopeptidase antibody
      see all

    Images

    • Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
      Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
      Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/5000 dilution (purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 107 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
      Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
      All lanes : Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/5000 dilution (purified)

      Lane 1 : Mouse heart lysates
      Lane 2 : Rat spleen lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 107 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling ARTS1/ERAP1 with Purified ab124669 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
      Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
      All lanes : Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/1000 dilution (unpurified)

      Lane 1 : K562 cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP

      Predicted band size: 107 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

      Unpurified ab124669, at a 1/50 dilution, staining ARTS1/ERAP1 in paraffin embedded Human uterus tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
      Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)

    References (1)

    Publishing research using ab124669? Please let us know so that we can cite the reference in this datasheet.

    ab124669 has been referenced in 1 publication.

    • Wang B  et al. p53 increases MHC class I expression by upregulating the endoplasmic reticulum aminopeptidase ERAP1. Nat Commun 4:2359 (2013). WB . PubMed: 23965983

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