Recombinant Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-ARTS1/ERAP1 antibody [EPR6069]
See all ARTS1/ERAP1 primary antibodies -
Description
Rabbit monoclonal [EPR6069] to ARTS1/ERAP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human ARTS1/ERAP1 aa 900-1000 (C terminal).
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Positive control
- WB: K562, HUVEC, mouse heart, rat spleen and A431 cell lysates. IHC-P: Human uterus and human bladder carcinoma tissue. Flow Cyt: K562 cells.
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General notes
This product was previously labelled as ARTS1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6069 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab124669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 107 kDa. | |
| IHC-P | 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/50 - 1/100.
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Target
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Function
Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the peptidase M1 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 51752 Human
- Entrez Gene: 80898 Mouse
- Entrez Gene: 80897 Rat
- Omim: 606832 Human
- SwissProt: Q9NZ08 Human
- SwissProt: Q9EQH2 Mouse
- SwissProt: Q9JJ22 Rat
- Unigene: 716426 Human
see all -
Alternative names
- A LAP antibody
- A-LAP antibody
- Adipocyte derived leucine aminopeptidase antibody
see all
Images
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![Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)](https://www.abcam.com/ps/products/124/ab124669/Images/ab124669-298942-anti-arts1-antibody-epr6069-western-blot.jpg)
Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/5000 dilution (purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 107 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)](https://www.abcam.com/ps/products/124/ab124669/Images/ab124669-298937-anti-arts1-antibody-epr6069-western-blot.jpg)
Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)All lanes : Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/5000 dilution (purified)
Lane 1 : Mouse heart lysates
Lane 2 : Rat spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 107 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)](https://www.abcam.com/ps/products/124/ab124669/Images/ab124669-298362-anti-arts1-antibody-epr6069-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling ARTS1/ERAP1 with Purified ab124669 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)](https://www.abcam.com/ps/products/124/ab124669/Images/ab124669-138399-anti-arts1-antibody-epr6069-western-blot.jpg)
Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)All lanes : Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669) at 1/1000 dilution (unpurified)
Lane 1 : K562 cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP
Predicted band size: 107 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)](https://www.abcam.com/ps/products/124/ab124669/Images/ab124669-138398-anti-arts1-antibody-epr6069-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] (ab124669)Unpurified ab124669, at a 1/50 dilution, staining ARTS1/ERAP1 in paraffin embedded Human uterus tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Wang B et al. p53 increases MHC class I expression by upregulating the endoplasmic reticulum aminopeptidase ERAP1. Nat Commun 4:2359 (2013). WB . Read more (PubMed: 23965983) »
