Recombinant Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (ab232466)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free
See all ARTS1/ERAP1 primary antibodies -
Description
Rabbit monoclonal [EPR6069] to ARTS1/ERAP1 - BSA and Azide free -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human ARTS1/ERAP1 aa 900-1000 (C terminal).
Database link: Q9NZ08 -
Positive control
- IHC-P: Human bladder carcinoma tissue.
-
General notes
ab232466 is the carrier-free version of ab124669 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab232466 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as ARTS1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6069 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab232466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 107 kDa. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. We do not guarantee IHC-P for mouse and rat. |
Target
-
Function
Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the peptidase M1 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
-
Database links
- Entrez Gene: 51752 Human
- Entrez Gene: 80898 Mouse
- Entrez Gene: 80897 Rat
- Omim: 606832 Human
- SwissProt: Q9NZ08 Human
- SwissProt: Q9EQH2 Mouse
- SwissProt: Q9JJ22 Rat
- Unigene: 716426 Human
see all -
Alternative names
- A LAP antibody
- A-LAP antibody
- Adipocyte derived leucine aminopeptidase antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (ab232466)](https://www.abcam.com/ps/products/232/ab232466/Images/ab232466-327168-anti-arts1-antibody-epr6069-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (ab232466)Unpurified ab124669, at a 1/50 dilution, staining ARTS1/ERAP1 in paraffin embedded Human uterus tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124669).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (ab232466)](https://www.abcam.com/ps/products/232/ab232466/Images/ab232466-308851-anti-arts1-antibody-epr6069-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (ab232466)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling ARTS1/ERAP1 with Purified ab124669 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124669).
Datasheets and documents
References
ab232466 has not yet been referenced specifically in any publications.
