Recombinant Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (ab232466)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (ab232466)](https://www.abcam.com/ps/products/232/ab232466/Images/ab232466-327168-anti-arts1-antibody-epr6069-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (ab232466)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6069] to ARTS1/ERAP1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free
See all ARTS1/ERAP1 primary antibodies -
Description
Rabbit monoclonal [EPR6069] to ARTS1/ERAP1 - BSA and Azide free -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human bladder carcinoma tissue.
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General notes
ab232466 is the carrier-free version of ab124669.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6069 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 107 kDa.
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
We do not guarantee IHC-P for mouse and rat. |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 107 kDa. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. We do not guarantee IHC-P for mouse and rat. |
Target
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Function
Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the peptidase M1 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 51752 Human
- Entrez Gene: 80898 Mouse
- Entrez Gene: 80897 Rat
- Omim: 606832 Human
- SwissProt: Q9NZ08 Human
- SwissProt: Q9EQH2 Mouse
- SwissProt: Q9JJ22 Rat
- Unigene: 716426 Human
see all -
Alternative names
- A LAP antibody
- A-LAP antibody
- Adipocyte derived leucine aminopeptidase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (ab232466)
Unpurified ab124669, at a 1/50 dilution, staining ARTS1/ERAP1 in paraffin embedded Human uterus tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124669).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (ab232466)](https://www.abcam.com/ps/products/232/ab232466/Images/ab232466-308851-anti-arts1-antibody-epr6069-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (ab232466)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling ARTS1/ERAP1 with Purified ab124669 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124669).
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![Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (ab232466)](https://www.abcam.com/ps/products/232/ab232466/Images/ab232466-3-benefits-of-recombinant-antibodies.png)
Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (ab232466)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab232466 has been referenced in 1 publication.
- Seamon K et al. The Differential Expression of ERAP1/ERAP2 and Immune Cell Activation in Pre-eclampsia. Front Immunol 11:396 (2020). PubMed: 32210971
