Recombinant
RabMAb

Recombinant Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free (ab232466)

Overview

  • Product name

    Anti-ARTS1/ERAP1 antibody [EPR6069] - BSA and Azide free
    See all ARTS1/ERAP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6069] to ARTS1/ERAP1 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ARTS1/ERAP1 aa 900-1000 (C terminal).
    Database link: Q9NZ08

  • Positive control

    • IHC-P: Human bladder carcinoma tissue.
  • General notes

    ab232466 is the carrier-free version of ab124669 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232466 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as ARTS1

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 107 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse and rat.

Target

  • Function

    Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the peptidase M1 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A LAP antibody
    • A-LAP antibody
    • Adipocyte derived leucine aminopeptidase antibody
    • Adipocyte-derived leucine aminopeptidase antibody
    • ALAP antibody
    • Aminopeptidase PILS antibody
    • Aminopeptidase regulator of TNFR1 shedding antibody
    • APPILS antibody
    • ARTS 1 antibody
    • ARTS-1 antibody
    • Arts1 antibody
    • Endoplasmic reticulum aminopeptidase 1 antibody
    • Endoplasmic reticulum aminopeptidase associated with antigen processing antibody
    • ERAAP antibody
    • ERAAP1 antibody
    • ERAP 1 antibody
    • Erap1 antibody
    • ERAP1_HUMAN antibody
    • KIAA0525 antibody
    • kiaa0525 protein antibody
    • PILS AP antibody
    • PILS-AP antibody
    • PILSA antibody
    • PILSAP antibody
    • Puromycin insensitive leucyl specific aminopeptidase antibody
    • Puromycin-insensitive leucyl-specific aminopeptidase antibody
    • Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator antibody
    • type 1 tumor necrosis factor receptors shedding aminopeptidase regulator antibody
    • VEGF-induced aminopeptidase antibody
    see all

Images

  • Unpurified ab124669, at a 1/50 dilution, staining ARTS1/ERAP1 in paraffin embedded Human uterus tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124669).

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling ARTS1/ERAP1 with Purified ab124669 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124669).

References

ab232466 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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