Overview

  • Product name
    Anti-Aryl hydrocarbon Receptor antibody
    See all Aryl hydrocarbon Receptor primary antibodies
  • Description
    Goat polyclonal to Aryl hydrocarbon Receptor
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Aryl hydrocarbon Receptor aa 749-763 (internal sequence) (Cysteine residue). (NP_001612.1).
    Sequence:

    C-KHGLNPQSAIITPQT


    Database link: P35869

  • Positive control
    • WB: HeLa cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab223473 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 0.5 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 96 kDa).

Target

  • Function
    Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues.
  • Tissue specificity
    Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Cellular localization
    Cytoplasm. Nucleus. Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ah receptor antibody
    • AhR antibody
    • AHR_HUMAN antibody
    • Aromatic hydrocarbon receptor antibody
    • Aryl hydrocarbon receptor antibody
    • Aryl hydrocarbon receptor precursor antibody
    • bHLHe76 antibody
    • Class E basic helix loop helix protein 76 antibody
    • Class E basic helix-loop-helix protein 76 antibody
    • HGNC:348 antibody
    see all

Images

  • Anti-Aryl hydrocarbon Receptor antibody (ab223473) at 0.2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 96 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223473 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab223473.
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