Western blot abreview for Anti-Aryl hydrocarbon Receptor antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (embryonic stem cell)
Loading amount
200000 cells
Specification
embryonic stem cell
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

Other product details

Dilution
1/500
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: block

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 100 kDa
Positive control
lane 1

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Submitted Dec 14 2010

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