Flow Cytometry abreview for Anti-Aryl hydrocarbon Receptor antibody

Poor
Abreviews
Application
Flow Cytometry
Sample
Mouse Cell (embryonic stem cell)
Specification
embryonic stem cell
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: enzyme-free
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
cells

Other product details

Dilution
1/100
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: 0.2% BSA + 0.1% sodium azide in PBS

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit IgG-PE
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/200

Additional data

Additional Notes
purple plot is control, secondary antibody alone.
green plot is primary and secondary antibody.
Abcam response
Thank you for sharing this data with us. This antibody has not previously been tested in flow and it would appear from this abreview that it does not work in this application, under these conditions. Abcam would welcome feedback from other customers who have tried ab28698 in flow.

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Verified customer

Submitted Dec 17 2010

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