Overview

  • Product name
    Anti-Aryl hydrocarbon Receptor antibody
    See all Aryl hydrocarbon Receptor primary antibodies
  • Description
    Rabbit polyclonal to Aryl hydrocarbon Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, EMSA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide from the N terminal residues of human Aryl hydrocarbon Receptor (NM_001621).

  • Positive control
    • HepG2 cell lysate. Rat kidney.

Properties

  • Form
    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 0.184% Tris glycine
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1 - 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration. PubMed: 22287322
ICC/IF 1/1 - 1/100.
WB 1/200 - 1/500. Predicted molecular weight: 96 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues.
  • Tissue specificity
    Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Cellular localization
    Cytoplasm. Nucleus. Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ah receptor antibody
    • AhR antibody
    • AHR_HUMAN antibody
    • Aromatic hydrocarbon receptor antibody
    • Aryl hydrocarbon receptor antibody
    • Aryl hydrocarbon receptor precursor antibody
    • bHLHe76 antibody
    • Class E basic helix loop helix protein 76 antibody
    • Class E basic helix-loop-helix protein 76 antibody
    • HGNC:348 antibody
    see all

Images

  • Anti-Aryl hydrocarbon Receptor antibody (ab84833) at 1/200 dilution + HepG2 cell lysate

    Predicted band size: 96 kDa
    Observed band size: 96 kDa

  • ab84833, at 1/200 dilution, staining Aryl hydrocarbon Receptor in formalin-fixed paraffin-embedded rat kidney by Immunohistochemistry.
  • Flow cytometry using Aryl hydrocarbon Receptor antibody, ab84833. The histogram above shows ab84833 positive human embryonic stem cells (in green) and secondary only negative control (in purple).

    See Abreview

  • ab84833 staining Aryl hydrocarbon receptor in MDA-MB-231 cells treated with tranilast (ab120643), by ICC/IF. Increase in Aryl hydrocarbon receptor expression correlates with increased concentration of tranilast, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120643 (telmisartan) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab84833 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • ab84833 staining Aryl hydrocarbon Receptor in Human colon tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% sreum for 30 minutes at 21°C. Samples were incubated with primary antibody for 16 hours at 4°C. An undiluted Alexa fluor®555-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab84833 staining Aryl hydrocarbon receptor protein on mouse embryonic stem cell by ICC/IF. The cells were paraformaldehyde fixed, permeabilized in 0.1% triton-x and blocked in 1% serum for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with sample for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rabbit IgG, diluted 1/100 was used as secondary.

    See Abreview

References

This product has been referenced in:
  • Duan Z  et al. Nuclear localization of Newcastle disease virus matrix protein promotes virus replication by affecting viral RNA synthesis and transcription and inhibiting host cell transcription. Vet Res 50:22 (2019). Read more (PubMed: 30894203) »
  • Silveira LTR  et al. Protective effect of resveratrol on urogenital sinus and prostate development in rats exposed in utero to TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin). Reprod Toxicol N/A:N/A (2018). Read more (PubMed: 29935225) »
See all 7 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (colon)
Specification
colon
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Mar 01 2013

Application
Western blot
Sample
Human Tissue lysate - whole (human colon lysate)
Loading amount
40 µg
Specification
human colon lysate
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Feb 09 2012

Answer

Thank you for your inquiry. I can confirm that the flow cytometry image with ab84833 is courtesy of an anonymous Abreview. I would like to suggest to review the Abreview: https://www.abcam.com/index.html?datasheet=84833&tab=abreviews&intabreviewid=21732 If you have any additional questions about the specific protocol used I would like to encourage you to contact the author of the Abreview via the link provided on the top of the page with the abreview. Protocols usually have to be optimized and I would like to recommend to start with a standard protocol for intracellular staining: https://www.abcam.com/index.html?pageconfig=resource&rid=11448 I hope this information is helpful. Please do not hesitate to contact me with any further questions.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic stem cell)
Specification
embryonic stem cell
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1 % triton-x
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 24 2010

Application
Flow Cytometry
Sample
Mouse Cell (embryonic stem cell)
Specification
embryonic stem cell
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: enzyme free
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
cells

Abcam user community

Verified customer

Submitted Nov 23 2010

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