Arylamine N-acetyltransferase Activity Assay Kit (Fluorometric) (ab284533)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Sample type: Purified protein
Overview
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Product name
Arylamine N-acetyltransferase Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Purified protein -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat, Human -
Product overview
Arylamine N-acetyltransferase Activity Assay Kit (Fluorometric) (ab284533) (K2031) is a simple, one step plate based assay that measures both NAT1 and NAT2 activity in tissue samples, S9 fractions, recombinant enzyme or purified protein samples.
This kit can detect as low as 1 µU in samples.
General range: 0-500 pmol/well of acetylated standard.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K2031 Arylamine N-acetyltransferase Activity Assay Kit (Fluorometric). K2031-100 is the same size as the 100 test size of ab284533.
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Tested applications
Suitable for: Enzyme activity assaymore details -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Acetylated Standard 1 x 50µl DTT 1 x 100µl NAT Assay Buffer 1 x 25ml NAT Positive Control 1 vial NAT Substrate I 1 x 200µl NAT Substrate II 2 vials -
Research areas
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Function
Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens. -
Sequence similarities
Belongs to the arylamine N-acetyltransferase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- AAC1
- ARY1_HUMAN
- Arylamide acetylase 1
see all -
Database links
- Entrez Gene: 9 Human
- Entrez Gene: 116631 Rat
- Omim: 108345 Human
- SwissProt: P18440 Human
- SwissProt: P50297 Rat
- Unigene: 2 Human
- Unigene: 591847 Human
- Unigene: 37420 Rat
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab284533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Enzyme activity assay |
Use at an assay dependent concentration.
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Notes |
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Enzyme activity assay
Use at an assay dependent concentration. |
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab284533 has not yet been referenced specifically in any publications.