Anti-AS160 antibody (ab134749)
Key features and details
- Rabbit polyclonal to AS160
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AS160 antibody
See all AS160 primary antibodies -
Description
Rabbit polyclonal to AS160 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig, Chimpanzee, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human AS160 aa 1248-1298 (C terminal).
Sequence:IRT LEQEKMAYQK TVEQLRKLLP ADALVNCDLL LRDLNCNPNN KAKIGNKP
Database link: NP_055647.2 -
Positive control
- Whole cell lysate from HeLa, 293T and Jurkat cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7.0 to 8.0 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab134749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 146 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. -
Tissue specificity
Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis. -
Sequence similarities
Contains 2 PID domains.
Contains 1 Rab-GAP TBC domain. -
Post-translational
modificationsPhosphorylated by AKT1; insulin-induced.
Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients. -
Cellular localization
Cytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells. - Information by UniProt
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Database links
- Entrez Gene: 9882 Human
- Omim: 612465 Human
- SwissProt: O60343 Human
- Unigene: 173802 Human
- Unigene: 210891 Human
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Alternative names
- Acrg embryonic lethality (mouse) minimal region ortholog antibody
- Acrg embryonic lethality minimal region ortholog antibody
- Acrg embryonic lethality mouse minimal region ortholog antibody
see all
Images
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Western blot - Anti-AS160 antibody (ab134749)All lanes : Anti-AS160 antibody (ab134749) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 50 µg
Lane 4 : Whole cell lysate from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 146 kDa
Exposure time: 10 seconds
Western Blot Gel 4-8% -
Immunoprecipitation - Anti-AS160 antibody (ab134749)Lane 1: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of AS160.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an upstream epitope of AS160.
Lane 3: Detection of Human AS160 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab134749 at 6 µg/mg lysate for IP.
Lane 4: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab134749 at 1 µg/ml.
Western Blot Gel 4-8%
Developed using the ECL technique with an exposure time of 3 seconds.
Protocols
Datasheets and documents
References (0)
ab134749 has not yet been referenced specifically in any publications.
