Product nameAnti-AS160 antibody
See all AS160 primary antibodies
DescriptionRabbit polyclonal to AS160
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: DogDoes not react with: Mouse, Rat
Synthetic peptide corresponding to Human AS160 aa 1250 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in human skeletal muscle tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab24469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 147 kDa).|
|IP||Use at an assay dependent concentration. PubMed: 20937822|
|IHC-P||1/135. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionMay act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Tissue specificityWidely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis.
Sequence similaritiesContains 2 PID domains.
Contains 1 Rab-GAP TBC domain.
modificationsPhosphorylated by AKT1; insulin-induced.
Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
Cellular localizationCytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
- Information by UniProt
- Acrg embryonic lethality (mouse) minimal region ortholog antibody
- Acrg embryonic lethality minimal region ortholog antibody
- Acrg embryonic lethality mouse minimal region ortholog antibody
All lanes : Anti-AS160 antibody (ab24469) at 0.46 µg/ml
Lane 1 : Human skeletal muscle cell lysate at 10 µg
Lane 2 : Human skeletal muscle cell lysate at 20 µg
Lane 3 : Human skeletal muscle cell lysate at 40 µg
Lane 4 : Human skeletal muscle cell lysate at 60 µg
All lanes : Anti-rabbit HRP
Predicted band size: 147 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Incubation with the primary antibody was at 4 degrees celsius overnight. The secondary anti-rabbit HRP antibody was incubated for one hour at room temperature.
Image courtesy of Human Protein Atlas
ab24469 staining AS160 in human stomach. Paraffin embedded human stomach tissue was incubated with ab24469 (1/135 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab24469 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
Anti-AS160 antibody (ab24469) at 1 µg/ml + AS160 Human Partial Recombinant Protein (His Tagged) at 0.1 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 147 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Gudiksen A & Pilegaard H PGC-1a and fasting-induced PDH regulation in mouse skeletal muscle. Physiol Rep 5:N/A (2017). WB ; Mouse . Read more (PubMed: 28400503) »
- Kim TY et al. Substrate trapping proteomics reveals targets of the ßTrCP2/FBXW11 ubiquitin ligase. Mol Cell Biol 35:167-81 (2015). WB ; Human . Read more (PubMed: 25332235) »