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  1. Link

    as160-phospho-s588-antibody-ab65754.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other
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Anti-AS160 (phospho S588) antibody (ab65754)

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  • Western blot - Anti-AS160 (phospho S588) antibody (ab65754)
  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-AS160 (phospho S588) antibody
    See all AS160 primary antibodies
  • Description

    Sheep polyclonal to AS160 (phospho S588)
  • Host species

    Sheep
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human AS160 aa 583-593 conjugated to keyhole limpet haemocyanin.
    Sequence:

    CRGRLGSVDSFE

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Tagged AS160 wild type, S588A mutant, and T642A mutant over-expressed transiently in HEK293 cells, basal or with 1 µM insulin treatment for 15 minutes.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.50
    Preservative: 0.05% Sodium azide
    Constituents: 0.238% HEPES, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other

Associated products

  • Isotype control

    • Sheep IgG - Isotype Control (ab37385)

Applications

Our Abpromise guarantee covers the use of ab65754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 147 kDa.

Target

  • Function

    May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
  • Tissue specificity

    Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis.
  • Sequence similarities

    Contains 2 PID domains.
    Contains 1 Rab-GAP TBC domain.
  • Post-translational
    modifications

    Phosphorylated by AKT1; insulin-induced.
    Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
    Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
  • Cellular localization

    Cytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
  • Target information above from: UniProt accession O60343 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9882 Human
    • Entrez Gene: 210789 Mouse
    • Omim: 612465 Human
    • SwissProt: O60343 Human
    • SwissProt: Q8BYJ6 Mouse
    • Unigene: 173802 Human
    • Unigene: 210891 Human
    • Unigene: 267385 Mouse
    • Unigene: 320639 Mouse
    see all
  • Alternative names

    • Acrg embryonic lethality (mouse) minimal region ortholog antibody
    • Acrg embryonic lethality minimal region ortholog antibody
    • Acrg embryonic lethality mouse minimal region ortholog antibody
    • Akt substrate of 160 kDa antibody
    • AS 160 antibody
    • AS160 antibody
    • BUB2 antibody
    • CDC16 antibody
    • KIAA0603 antibody
    • NIDDM5 antibody
    • TBC (Tre 2 BUB2 CDC16) domain containing protein antibody
    • TBC Tre 2 BUB2 CDC16 domain containing protein antibody
    • TBC1 D4 antibody
    • TBC1 domain family member 4 antibody
    • Tbc1d4 antibody
    • TBCD4_HUMAN antibody
    • Tre-2 antibody
    see all

Images

  • Western blot - Anti-AS160 (phospho S588) antibody (ab65754)
    Western blot - Anti-AS160 (phospho S588) antibody (ab65754)
    Western blot analysis of immunoprecipitated Flagtagged AS160 wild type (WT), S588A mutant (S588A), and T642A mutant (T642A) over-expressed transiently in HEK293 cells, basal (-) or with (+) 1 µM insulin treatment for 15 minutes using ab65754 at 2ug/ml.

Protocols

  • Western blot protocols

Datasheets and documents

    • Datasheet
  • References

    ab65754 has not yet been referenced specifically in any publications.

    Publishing research using ab65754? Please let us know so that we can cite the reference in this datasheet.

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