Key features and details
- Sheep polyclonal to AS160 (phospho S588)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-AS160 (phospho S588) antibody
See all AS160 primary antibodies
DescriptionSheep polyclonal to AS160 (phospho S588)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
- Tagged AS160 wild type, S588A mutant, and T642A mutant over-expressed transiently in HEK293 cells, basal or with 1 µM insulin treatment for 15 minutes.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 0.238% HEPES, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 147 kDa.|
FunctionMay act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Tissue specificityWidely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis.
Sequence similaritiesContains 2 PID domains.
Contains 1 Rab-GAP TBC domain.
modificationsPhosphorylated by AKT1; insulin-induced.
Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
Cellular localizationCytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
- Information by UniProt
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ab65754 has not yet been referenced specifically in any publications.