Anti-AS160 (phospho S588) antibody (ab65754)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-AS160 (phospho S588) antibody
See all AS160 primary antibodies -
Description
Sheep polyclonal to AS160 (phospho S588) -
Host species
Sheep -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human AS160 aa 583-593 conjugated to keyhole limpet haemocyanin.
Sequence:CRGRLGSVDSFE
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Positive control
- Tagged AS160 wild type, S588A mutant, and T642A mutant over-expressed transiently in HEK293 cells, basal or with 1 µM insulin treatment for 15 minutes.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 0.238% HEPES, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab65754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Predicted molecular weight: 147 kDa. |
Target
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Function
May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. -
Tissue specificity
Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis. -
Sequence similarities
Contains 2 PID domains.
Contains 1 Rab-GAP TBC domain. -
Post-translational
modificationsPhosphorylated by AKT1; insulin-induced.
Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients. -
Cellular localization
Cytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells. - Information by UniProt
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Database links
- Entrez Gene: 9882 Human
- Entrez Gene: 210789 Mouse
- Omim: 612465 Human
- SwissProt: O60343 Human
- SwissProt: Q8BYJ6 Mouse
- Unigene: 173802 Human
- Unigene: 210891 Human
- Unigene: 267385 Mouse
see all -
Alternative names
- Acrg embryonic lethality (mouse) minimal region ortholog antibody
- Acrg embryonic lethality minimal region ortholog antibody
- Acrg embryonic lethality mouse minimal region ortholog antibody
see all
Images
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Western blot - Anti-AS160 (phospho S588) antibody (ab65754)Western blot analysis of immunoprecipitated Flagtagged AS160 wild type (WT), S588A mutant (S588A), and T642A mutant (T642A) over-expressed transiently in HEK293 cells, basal (-) or with (+) 1 µM insulin treatment for 15 minutes using ab65754 at 2ug/ml.
Protocols
Datasheets and documents
References
ab65754 has not yet been referenced specifically in any publications.
