Recombinant
RabMAb

Recombinant Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)

Overview

  • Product name

    Anti-AS160 (phospho T642) antibody [EPR2733(2)]
    See all AS160 primary antibodies
  • Description

    Rabbit monoclonal [EPR2733(2)] to AS160 (phospho T642)
  • Host species

    Rabbit
  • Specificity

    ab131214 only detects AS160 when phosphorylated at Threonine 642.
  • Tested applications

    Suitable for: Dot blot, WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human AS160 (phospho T642). The exact sequence is proprietary.
    Database link: O60343

  • Positive control

    • WB: HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate and 293T cell lysate - insulin-treated. Dot Blot: AS160 (phospho T642) phospho peptide.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 6.40 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2733(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 146 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
    • Tissue specificity

      Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis.
    • Sequence similarities

      Contains 2 PID domains.
      Contains 1 Rab-GAP TBC domain.
    • Post-translational
      modifications

      Phosphorylated by AKT1; insulin-induced.
      Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
      Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
    • Cellular localization

      Cytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • Acrg embryonic lethality (mouse) minimal region ortholog antibody
      • Acrg embryonic lethality minimal region ortholog antibody
      • Acrg embryonic lethality mouse minimal region ortholog antibody
      • Akt substrate of 160 kDa antibody
      • AS 160 antibody
      • AS160 antibody
      • BUB2 antibody
      • CDC16 antibody
      • KIAA0603 antibody
      • NIDDM5 antibody
      • TBC (Tre 2 BUB2 CDC16) domain containing protein antibody
      • TBC Tre 2 BUB2 CDC16 domain containing protein antibody
      • TBC1 D4 antibody
      • TBC1 domain family member 4 antibody
      • Tbc1d4 antibody
      • TBCD4_HUMAN antibody
      • Tre-2 antibody
      see all

    Images

    • All lanes : Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1.12 µg/ml

      Lane 1 : HEK-293 (human embryonic kidney epithelial cell) grown in serum free media overnight whole cell lysate
      Lane 2 : HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate
      Lane 3 : HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate. Then the membrane was incubated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 146 kDa



      Blocking and diluting buffer: 5% NFDM/TBST. 

    • Dot blot analysis of AS160 (phospho T642) phospho peptide (Lane 1) and AS160 non-phospho peptide (Lane 2) labelling AS160 (phospho T642) phospho peptide with ab131214 at a dilution of 1:1000 dilution (1.12μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1/1000 dilution

      Lane 1 : 293T cell lysate - untreated
      Lane 2 : 293T cell lysate - insulin-treated

      Predicted band size: 146 kDa



      Bottom pannel shows detection of total AS160 with a general anti AS160 antibody.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab131214 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Muscle fibers)
    Gel Running Conditions
    Reduced Denaturing (66-440 kDa separation matrix)
    Loading amount
    5 µg
    Specification
    Muscle fibers
    Blocking step
    ProteinSimple Blocking Buffer as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C

    Irene Tobias

    Verified customer

    Submitted Mar 19 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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