Recombinant
RabMAb

Recombinant Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)

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Overview

  • Product name

    Anti-AS160 (phospho T642) antibody [EPR2733(2)]
    See all AS160 primary antibodies

  • Description

    Rabbit monoclonal [EPR2733(2)] to AS160 (phospho T642)

  • Host species

    Rabbit

  • Specificity

    ab131214 only detects AS160 when phosphorylated at Threonine 642.

  • Tested applications

    Suitable for: Dot blot, WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human AS160 (phospho T642). The exact sequence is proprietary.
    Database link: O60343

  • Positive control

    • WB: HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate and 293T cell lysate - insulin-treated. Dot Blot: AS160 (phospho T642) phospho peptide.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 6.40 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR2733(2)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 146 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function

      May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

    • Tissue specificity

      Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis.

    • Sequence similarities

      Contains 2 PID domains.
      Contains 1 Rab-GAP TBC domain.

    • Post-translational
      modifications

      Phosphorylated by AKT1; insulin-induced.
      Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
      Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.

    • Cellular localization

      Cytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
    • Information by UniProt

    • Database links

      • Alternative names

        • Acrg embryonic lethality (mouse) minimal region ortholog antibody
        • Acrg embryonic lethality minimal region ortholog antibody
        • Acrg embryonic lethality mouse minimal region ortholog antibody
        • Akt substrate of 160 kDa antibody
        • AS 160 antibody
        • AS160 antibody
        • BUB2 antibody
        • CDC16 antibody
        • KIAA0603 antibody
        • NIDDM5 antibody
        • TBC (Tre 2 BUB2 CDC16) domain containing protein antibody
        • TBC Tre 2 BUB2 CDC16 domain containing protein antibody
        • TBC1 D4 antibody
        • TBC1 domain family member 4 antibody
        • Tbc1d4 antibody
        • TBCD4_HUMAN antibody
        • Tre-2 antibody
        see all

      Images

      • Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
        Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
        All lanes : Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1.12 µg/ml

        Lane 1 : HEK-293 (human embryonic kidney epithelial cell) grown in serum free media overnight whole cell lysate
        Lane 2 : HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate
        Lane 3 : HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate. Then the membrane was incubated with alkaline phosphatase

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 146 kDa



        Blocking and diluting buffer: 5% NFDM/TBST. 

      • Dot Blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
        Dot Blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)

        Dot blot analysis of AS160 (phospho T642) phospho peptide (Lane 1) and AS160 non-phospho peptide (Lane 2) labelling AS160 (phospho T642) phospho peptide with ab131214 at a dilution of 1:1000 dilution (1.12μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.

        Blocking and dilution buffer: 5% NFDM/TBST.

      • Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
        Western blot - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
        All lanes : Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1/1000 dilution

        Lane 1 : 293T cell lysate - untreated
        Lane 2 : 293T cell lysate - insulin-treated

        Predicted band size: 146 kDa



        Bottom pannel shows detection of total AS160 with a general anti AS160 antibody.

      • Other - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
        Other - Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab131214 has not yet been referenced specifically in any publications.

      Publishing research using ab131214? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Western blot abreview for Anti-AS160 (phospho T642) antibody [EPR2733(2)]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (Muscle fibers)
      Gel Running Conditions
      Reduced Denaturing (66-440 kDa separation matrix)
      Loading amount
      5 µg
      Specification
      Muscle fibers
      Blocking step
      ProteinSimple Blocking Buffer as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
      Read More

      Irene Tobias

      Verified customer

      Submitted Mar 19 2018

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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