Recombinant Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2733(2)] to AS160 (phospho T642)
- Suitable for: Dot blot, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AS160 (phospho T642) antibody [EPR2733(2)]
See all AS160 primary antibodies -
Description
Rabbit monoclonal [EPR2733(2)] to AS160 (phospho T642) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human AS160 (phospho T642). The exact sequence is proprietary.
Database link: O60343 -
Positive control
- WB: HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate and 293T cell lysate - insulin-treated. Dot Blot: AS160 (phospho T642) phospho peptide.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2733(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
1/1000.
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 146 kDa.
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Notes |
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Dot blot
1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 146 kDa. |
Target
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Function
May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. -
Tissue specificity
Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis. -
Sequence similarities
Contains 2 PID domains.
Contains 1 Rab-GAP TBC domain. -
Post-translational
modificationsPhosphorylated by AKT1; insulin-induced.
Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients. -
Cellular localization
Cytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells. - Information by UniProt
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Database links
- Entrez Gene: 9882 Human
- Omim: 612465 Human
- SwissProt: O60343 Human
- Unigene: 210891 Human
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Alternative names
- Acrg embryonic lethality (mouse) minimal region ortholog antibody
- Acrg embryonic lethality minimal region ortholog antibody
- Acrg embryonic lethality mouse minimal region ortholog antibody
see all
Images
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All lanes : Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1.12 µg/ml
Lane 1 : HEK-293 (human embryonic kidney epithelial cell) grown in serum free media overnight whole cell lysate
Lane 2 : HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate
Lane 3 : HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 146 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Dot blot analysis of AS160 (phospho T642) phospho peptide (Lane 1) and AS160 non-phospho peptide (Lane 2) labelling AS160 (phospho T642) phospho peptide with ab131214 at a dilution of 1:1000 dilution (1.12μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.
Blocking and dilution buffer: 5% NFDM/TBST. -
All lanes : Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1/1000 dilution
Lane 1 : 293T cell lysate - untreated
Lane 2 : 293T cell lysate - insulin-treated
Predicted band size: 146 kDaBottom pannel shows detection of total AS160 with a general anti AS160 antibody.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab131214 has been referenced in 1 publication.
- Tobias IS et al. Sex and fiber type independently influence AMPK, TBC1D1, and TBC1D4 at rest and during recovery from high-intensity exercise in humans. J Appl Physiol (1985) 128:350-361 (2020). PubMed: 31895596