Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2733(2)] to AS160 (phospho T642)
- Suitable for: Dot blot, WB
- Reacts with: Human
Product nameAnti-AS160 (phospho T642) antibody [EPR2733(2)]
See all AS160 primary antibodies
DescriptionRabbit monoclonal [EPR2733(2)] to AS160 (phospho T642)
Specificityab131214 only detects AS160 when phosphorylated at Threonine 642.
Tested applicationsSuitable for: Dot blot, WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human AS160 (phospho T642). The exact sequence is proprietary.
Database link: O60343
- WB: HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate and 293T cell lysate - insulin-treated. Dot Blot: AS160 (phospho T642) phospho peptide.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 6.40 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 146 kDa.|
FunctionMay act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Tissue specificityWidely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis.
Sequence similaritiesContains 2 PID domains.
Contains 1 Rab-GAP TBC domain.
modificationsPhosphorylated by AKT1; insulin-induced.
Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
Cellular localizationCytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
- Information by UniProt
- Acrg embryonic lethality (mouse) minimal region ortholog antibody
- Acrg embryonic lethality minimal region ortholog antibody
- Acrg embryonic lethality mouse minimal region ortholog antibody
All lanes : Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1.12 µg/ml
Lane 1 : HEK-293 (human embryonic kidney epithelial cell) grown in serum free media overnight whole cell lysate
Lane 2 : HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate
Lane 3 : HEK-293 grown in serum free media overnight, then treated with 100nM Calyculin A (ab141784) for 50min and then 100ng/ml Insulin was added for the last 20min, whole cell lysate. Then the membrane was incubated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 146 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
Dot blot analysis of AS160 (phospho T642) phospho peptide (Lane 1) and AS160 non-phospho peptide (Lane 2) labelling AS160 (phospho T642) phospho peptide with ab131214 at a dilution of 1:1000 dilution (1.12μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes : Anti-AS160 (phospho T642) antibody [EPR2733(2)] (ab131214) at 1/1000 dilution
Lane 1 : 293T cell lysate - untreated
Lane 2 : 293T cell lysate - insulin-treated
Predicted band size: 146 kDa
Bottom pannel shows detection of total AS160 with a general anti AS160 antibody.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab131214 has not yet been referenced specifically in any publications.