Recombinant
RabMAb

Recombinant Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free (ab218844)

Overview

  • Product name

    Anti-AS160 (phospho T642) antibody [EPR2733(2)] - BSA and Azide free
    See all AS160 primary antibodies
  • Description

    Rabbit monoclonal [EPR2733(2)] to AS160 (phospho T642) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects AS160 when phosphorylated at Threonine 642.

  • Tested applications

    Suitable for: Dot blot, WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human AS160 (phospho T642). The exact sequence is proprietary.
    Database link: O60343

  • Positive control

    • 293T cell lysate (insulin-treated)
  • General notes

    ab218844 is the carrier-free version of ab131214 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab218844 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 6.40 x 10 -11 M
    Learn more about KD
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2733(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab218844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 146 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
    • Tissue specificity

      Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis.
    • Sequence similarities

      Contains 2 PID domains.
      Contains 1 Rab-GAP TBC domain.
    • Post-translational
      modifications

      Phosphorylated by AKT1; insulin-induced.
      Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
      Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
    • Cellular localization

      Cytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • Acrg embryonic lethality (mouse) minimal region ortholog antibody
      • Acrg embryonic lethality minimal region ortholog antibody
      • Acrg embryonic lethality mouse minimal region ortholog antibody
      • Akt substrate of 160 kDa antibody
      • AS 160 antibody
      • AS160 antibody
      • BUB2 antibody
      • CDC16 antibody
      • KIAA0603 antibody
      • NIDDM5 antibody
      • TBC (Tre 2 BUB2 CDC16) domain containing protein antibody
      • TBC Tre 2 BUB2 CDC16 domain containing protein antibody
      • TBC1 D4 antibody
      • TBC1 domain family member 4 antibody
      • Tbc1d4 antibody
      • TBCD4_HUMAN antibody
      • Tre-2 antibody
      see all

    References

    ab218844 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab218844.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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