Key features and details
- Rabbit polyclonal to AS3
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-AS3 antibody
See all AS3 primary antibodies
DescriptionRabbit polyclonal to AS3
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to Human AS3.
- HeLa, 293T and NIH3T3 whole cell lysates.
Protein previously labeled as PDS5B.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70298 was affinity purified using an epitope specific to AS3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab70298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 165 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevancePDS5B, also known as APRIN (Androgen induced proliferation inhibitor) plays a role in androgen-induced proliferative arrest in prostate cells and is required for maintenance of sister chromatid cohesion during mitosis.
- AI646570 antibody
- Androgen induced proliferation inhibitor antibody
- Androgen-induced prostate proliferative shutoff associated protein AS3 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and prostate carcinoma (right) tissues labelling AS3 with ab70298 at 1/200 (1µg/ml) and 1/80 (2.5µg/ml). Detection: DAB and DyLight® 594-conjugated goat anti-rabbit IgG (1/100).
All lanes : Anti-AS3 antibody (ab70298) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 165 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Western Blot showing ab70298 at 0.1µg/ml staining AS3 in HeLa whole cell lysates immunoprecipitated using ab70298 at 3µg/mg lysate (1 mg/IP loaded/lane).
ab70298 has been referenced in 1 publication.
- Meisenberg C et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). PubMed: 30554942