Key features and details
- Rabbit polyclonal to ASAH1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ASAH1 antibody
See all ASAH1 primary antibodies
DescriptionRabbit polyclonal to ASAH1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 197-395 of Human ASAH1 (Uniprot Q13510).
- NT2D1 whole cell lysates, Human adenocarcinoma tissue
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 45 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively use Tris-EDTA buffer (pH8.0).|
FunctionHydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
Tissue specificityBroadly expressed with highest expression in heart.
Involvement in diseaseDefects in ASAH1 are the cause of Farber lipogranulomatosis (FL) [MIM:228000]; also known as Farber disease (FD). This sphingolipid disease is characterized by subcutaneous lipid-loaded nodules, excruciating pain in the joints and extremities, marked accumulation of ceramide in lysosomes, and death by three years of age.
Sequence similaritiesBelongs to the acid ceramidase family.
- Information by UniProt
- AC antibody
- ACDase antibody
- Acid CDase antibody
ab154401 has not yet been referenced specifically in any publications.