Key features and details
- Rabbit polyclonal to ASAH1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ASAH1 antibody
See all ASAH1 primary antibodies
DescriptionRabbit polyclonal to ASAH1
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide of 16 amino acids taken from near the C-terminus of human ASAH1. (EAW63795).
- Mouse heart tissue lysate
Multiple isoforms of ASAH1 are known to exist.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 52 kDa.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 2.5 µg/ml.|
FunctionHydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
Tissue specificityBroadly expressed with highest expression in heart.
Involvement in diseaseDefects in ASAH1 are the cause of Farber lipogranulomatosis (FL) [MIM:228000]; also known as Farber disease (FD). This sphingolipid disease is characterized by subcutaneous lipid-loaded nodules, excruciating pain in the joints and extremities, marked accumulation of ceramide in lysosomes, and death by three years of age.
Sequence similaritiesBelongs to the acid ceramidase family.
- Information by UniProt
- AC antibody
- ACDase antibody
- Acid CDase antibody
Lane 1 : Anti-ASAH1 antibody (ab74469) at 1 µg/ml
Lane 2 : Anti-ASAH1 antibody (ab74469) at 2 µg/ml
All lanes : Mouse heart tissue lysate
Lysates/proteins at 15 µg per lane.
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemical analysis of of ASAH1 expression in rat heart tissue with ab74469 at 20µg/ml.
ab74469 at 2.5µg/ml staining ASAH1 in human heart tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Immunofluorescence of ASAH1 in Human Heart cells using ab74469 at 20 ug/ml.
ab74469 has been referenced in 1 publication.
- Braun F et al. Altered lipid metabolism in the aging kidney identified by three layered omic analysis. Aging (Albany NY) 8:441-57 (2016). IHC . PubMed: 26886165