Product nameAnti-ASAH1 antibody [EPR12108]
See all ASAH1 primary antibodies
DescriptionRabbit monoclonal [EPR12108] to ASAH1
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human ASAH1 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q13510
- Human fetal lung and skin lysates; Human heart tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 45 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody is recommended.
FunctionHydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
Tissue specificityBroadly expressed with highest expression in heart.
Involvement in diseaseDefects in ASAH1 are the cause of Farber lipogranulomatosis (FL) [MIM:228000]; also known as Farber disease (FD). This sphingolipid disease is characterized by subcutaneous lipid-loaded nodules, excruciating pain in the joints and extremities, marked accumulation of ceramide in lysosomes, and death by three years of age.
Sequence similaritiesBelongs to the acid ceramidase family.
- Information by UniProt
- AC antibody
- ACDase antibody
- Acid CDase antibody
All lanes : Anti-ASAH1 antibody [EPR12108] (ab174828) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human skin lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling ASAH1 using ab174828 at a 1/50 dilution.
ab174828 has not yet been referenced specifically in any publications.