1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody is recommended.
Is unsuitable for ICC/IF or IP.
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
Broadly expressed with highest expression in heart.
Involvement in disease
Defects in ASAH1 are the cause of Farber lipogranulomatosis (FL) [MIM:228000]; also known as Farber disease (FD). This sphingolipid disease is characterized by subcutaneous lipid-loaded nodules, excruciating pain in the joints and extremities, marked accumulation of ceramide in lysosomes, and death by three years of age.