Overview

  • Product name
    Anti-ASAH1 antibody [EPR12108]
    See all ASAH1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12108] to ASAH1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ASAH1 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q13510

  • Positive control
    • Human fetal lung and skin lysates; Human heart tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12108
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 45 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The use of an HRP/AP polymerized secondary antibody is recommended.

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
    • Tissue specificity
      Broadly expressed with highest expression in heart.
    • Involvement in disease
      Defects in ASAH1 are the cause of Farber lipogranulomatosis (FL) [MIM:228000]; also known as Farber disease (FD). This sphingolipid disease is characterized by subcutaneous lipid-loaded nodules, excruciating pain in the joints and extremities, marked accumulation of ceramide in lysosomes, and death by three years of age.
    • Sequence similarities
      Belongs to the acid ceramidase family.
    • Cellular localization
      Lysosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • AC antibody
      • ACDase antibody
      • Acid CDase antibody
      • Acid ceramidase antibody
      • Acid ceramidase precursor antibody
      • Acid ceramidase subunit beta antibody
      • Acylsphingosine deacylase antibody
      • ASAH 1 antibody
      • ASAH antibody
      • ASAH1 antibody
      • ASAH1_HUMAN antibody
      • FLJ21558 antibody
      • FLJ22079 antibody
      • N acylsphingosine amidohydrolase (acid ceramidase) 1 antibody
      • N acylsphingosine amidohydrolase 1 antibody
      • N acylsphingosine amidohydrolase antibody
      • N-acylsphingosine amidohydrolase antibody
      • N-acylsphingosine deacylase antibody
      • PHP antibody
      • PHP32 antibody
      • Putative 32 kDa heart protein antibody
      • SMAPME antibody
      see all

    Images

    • All lanes : Anti-ASAH1 antibody [EPR12108] (ab174828) at 1/1000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : Human skin lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 45 kDa

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling ASAH1 using ab174828 at a 1/50 dilution.

    References

    ab174828 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174828.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up