1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May be involved in the cell-cell adhesion. May play a role in adipocyte differentiation and development of obesity.
Predominantly expressed in epithelial cells within different tissues and in the white adipose tissue. Expressed at high levels in small intestine and placenta, at intermediate levels in the heart, skeletal muscle, colon, spleen, kidney and lung and at low levels in the liver and peripheral blood leukocytes.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue, labeling ASAM with ab171552 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.