ION Potassium Green-2 AM, K+ indicator (ab142806)
Key features and details
- Cell membrane permeable K+ indicator
- Purity: > 90%
- Soluble in DMSO to 1mg/ml
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
ION Potassium Green-2 AM, K+ indicator -
Description
Cell membrane permeable K+ indicator -
Biological description
Cell membrane permeable K+ indicator. Related product (ab142807). APG-2 AM works in cells that have non-specific esterases to hydrolyse the AM to release the dye. Fluorescent probe.
We do not guarantee successful labelling of plant cells; optimisations to allow for loading into plant cells may be required
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Purity
> 90% -
General notes
ab142806 has low selectivity of Potassium over Sodium and the presence of Sodium will affect results.
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Chemical structure
Properties
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Excitation
526nm -
Emission
546nm -
Molecular weight
1128.02 -
Molecular formula
C55H64Cl2N2O19 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 1mg/ml -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
For more information on AM esters please visit our AM esters FAQ page.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(=O)OCOC(=O)CCC6=CC=2C(Oc1c(cc(CCC(=O)OCOC(C)=O)c(OCOC(C)=O)c1Cl)C=2c3ccc(c(OC)c3)N4CCOCCOCCN(CCOCCOCC4)c5ccc(C)cc5OC)=C(Cl)C6=O -
Source
Synthetic
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Research areas
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (8)
ab142806 has been referenced in 8 publications.
- Wang Y et al. Inhibition of sphingosine-1-phosphate receptor 3 suppresses ATP-induced NLRP3 inflammasome activation in macrophages via TWIK2-mediated potassium efflux. Front Immunol 14:1090202 (2023). PubMed: 36798132
- Smer-Barreto V et al. Discovery of senolytics using machine learning. Nat Commun 14:3445 (2023). PubMed: 37301862
- Hortle E et al. OXSR1 inhibits inflammasome activation by limiting potassium efflux during mycobacterial infection. Life Sci Alliance 5:N/A (2022). PubMed: 35545295
- Lee HB et al. Accumulation patterns of intracellular salts in a new halophilic amoeboflagellate, Euplaesiobystra salpumilio sp. nov., (Heterolobosea; Discoba) under hypersaline conditions. Front Microbiol 13:960621 (2022). PubMed: 35992684
- Wong F et al. Cytoplasmic condensation induced by membrane damage is associated with antibiotic lethality. Nat Commun 12:2321 (2021). PubMed: 33875652
- Camilli G et al. ß-Glucan-induced reprogramming of human macrophages inhibits NLRP3 inflammasome activation in cryopyrinopathies. J Clin Invest 130:4561-4573 (2020). PubMed: 32716363
- Ren G et al. ABRO1 promotes NLRP3 inflammasome activation through regulation of NLRP3 deubiquitination. EMBO J 38:N/A (2019). PubMed: 30787184
- Sekar P et al. Coordinate effects of P2X7 and extracellular acidification in microglial cells. Oncotarget 9:12718-12731 (2018). PubMed: 29560104