Overview

  • Product name

  • Description

    Rabbit polyclonal to ASAP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human ASAP2 aa 5-194 (N terminal).
    Database link: O43150-2

  • Positive control

    • 293T, A431, Jurkat, Raji and Mouse brain whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 107 kDa.

Target

  • Function

    Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration.
  • Tissue specificity

    Detected in heart, brain, placenta, kidney, monocytes and pancreas.
  • Sequence similarities

    Contains 2 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Domain

    The conserved Arg-464 in the Arf-GAP domain probably becomes part of the active site of bound small GTPases and is necessary for GTP hydrolysis.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues by SRC and PTK2B.
  • Cellular localization

    Cytoplasm. Golgi apparatus > Golgi stack membrane. Cell membrane. Colocalizes with F-actin and ARF6 in phagocytic cups.
  • Information by UniProt
  • Database links

  • Alternative names

    • AMAP 2 antibody
    • AMAP2 antibody
    • ANK repeat and PH domain-containing protein 2 antibody
    • Arf-GAP with SH3 domain antibody
    • ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 antibody
    • ASAP2 antibody
    • ASAP2_HUMAN antibody
    • centaurin, beta 3 antibody
    • CENTB3 antibody
    • DDEF 2 antibody
    • DDEF2 antibody
    • Development and differentiation enhancing factor 2 antibody
    • Development and differentiation-enhancing factor 2 antibody
    • FLJ42910 antibody
    • KIAA0400 antibody
    • PAG 3 antibody
    • PAG3 antibody
    • Pap alpha antibody
    • PAP antibody
    • Paxillin associated protein with ARFGAP activity 3 antibody
    • Paxillin-associated protein with ARF GAP activity 3 antibody
    • Pyk2 C terminus associated protein antibody
    • Pyk2 C-terminus-associated protein antibody
    • SHAG 1 antibody
    • SHAG1 antibody
    see all

Images

  • All lanes : Anti-ASAP2 antibody (ab137514) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 107 kDa



    7.5% SDS PAGE
  • Anti-ASAP2 antibody (ab137514) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 107 kDa



    7.5% SDS PAGE

References

ab137514 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab137514.
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