Overview

  • Product name

    Anti-ASB2 antibody
  • Description

    Rabbit polyclonal to ASB2
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of total ASB2 protein.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment (GST-tag) corresponding to Human ASB2 (internal sequence). Gene Accession: BC032354.
    Database link: Q96Q27

  • Positive control

    • Human thyroid cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab200370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Relevance

    The ankyrin repeat and SOCS box containing (ASB) protein family is comprised of members which each contain "SOCS boxes" but differ from other families of protein in the motifs they contain upstream of the SOCS box. SOCS boxes are carboxy terminal regions of homology found in the suppressor of cytokine signaling family of proteins. The box region is thought to be the point of interaction between SOCS proteins and E3 ubiquitin ligases. The SOCS box serves to couple suppressor of cytokine signalling (SOCS) proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation. The gene is induced by all trans retinoic acid. In myeloid leukemia cells, the expression of this encoded protein has been shown to induce growth inhibition and chromatin condensation. Multiple alternatively spliced transcript variants have been described for this gene but their full length sequences are not known.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • Ankyrin repeat and SOCS box 2 containing protein antibody
    • Ankyrin repeat and SOCS box containing 2 antibody
    • Ankyrin repeat and SOCS box containing protein 2 antibody
    • Ankyrin repeat and SOCS box containing protein 2a antibody
    • Ankyrin repeat and SOCS box protein 2 antibody
    • Ankyrin repeat containing protein ASB2 antibody
    • ASB 2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue labeling ASB2 with ab200370 at a 1/50 dilution.

References

ab200370 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryo)
Permeabilization
Yes - 0.3% TritonX-100, 15 min
Specification
embryo
Blocking step
NDS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
no fixation

Abcam user community

Verified customer

Submitted Sep 05 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up