Overview

  • Product name
  • Description
    Rabbit polyclonal to ASB5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 48 and 329 of Human ASB5 (UniProt ID: Q8WWX0).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, Molt4 and Raji cell lysates; HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab151452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    ASB5 is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. It may be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It may play a role in the initiation of arteriogenesis.
  • Database links
  • Alternative names
    • Ankyrin repeat and SOCS box containing 5 antibody
    • Ankyrin repeat and SOCS box protein 5 antibody
    • ASB 5 antibody
    • SOCS box protein ASB 5 antibody

Images

  • All lanes : Anti-ASB5 antibody (ab151452) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa S3 whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 36 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HepG2 cells labeling ASB5 with ab151452 at 1/500 dilution. The lower image was costained with Hoescht 33342.

References

ab151452 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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